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A mixture of anatase and rutile TiO 2nanoparticles induces histamine secretion in mast cells
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نویسنده
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chen e.y. ,garnica m. ,wang y.-c. ,mintz a.j. ,chen c.-s. ,chin w.-c.
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منبع
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particle and fibre toxicology - 2012 - دوره : 9 - شماره : 0
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چکیده
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Background: histamine released from mast cells,through complex interactions involving the binding of ige to fcεri receptors and the subsequent intracellular ca 2+signaling,can mediate many allergic/inflammatory responses. the possibility of titanium dioxide nanoparticles (tio 2nps),a nanomaterial pervasively used in nanotechnology and pharmaceutical industries,to directly induce histamine secretion without prior allergen sensitization has remained uncertain.results: tio 2np exposure increased both histamine secretion and cytosolic ca 2+concentration ([ca 2+] c) in a dose dependent manner in rat rbl-2h3 mast cells. the increase in intracellular ca 2+levels resulted primarily from an extracellular ca 2+influx via membrane l-type ca 2+channels. unspecific ca 2+entry via tio 2np-instigated membrane disruption was demonstrated with the intracellular leakage of a fluorescent calcein dye. oxidative stress induced by tio 2nps also contributed to cytosolic ca 2+signaling. the plc-ip 3-ip 3receptor pathways and endoplasmic reticulum (er) were responsible for the sustained elevation of [ca 2+] cand histamine secretion.conclusion: our data suggests that systemic circulation of nps may prompt histamine release at different locales causing abnormal inflammatory diseases. this study provides a novel mechanistic link between environmental tio 2np exposure and allergen-independent histamine release that can exacerbate manifestations of multiple allergic responses. © 2012 chen et al; licensee biomed central ltd.
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کلیدواژه
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Ca 2+ signaling; Histamine release; Mast cell; TiO 2 nanoparticles
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آدرس
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bioengineering,university of california at merced,merced,ca,united states,5200 north lake rd,merced,ca 95343, United States, bioengineering,university of california at merced,merced,ca,united states,5200 north lake rd,merced,ca 95343, United States, bioengineering,university of california at merced,merced,ca,united states,5200 north lake rd,merced,ca 95343, United States, bioengineering,university of california at merced,merced,ca,united states,5200 north lake rd,merced,ca 95343, United States, bioengineering,university of california at merced,merced,ca,united states,5200 north lake rd,merced,ca 95343, United States, bioengineering,university of california at merced,merced,ca,united states,5200 north lake rd,merced,ca 95343, United States
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Authors
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