Proteomic analysis of chicken embryonic trachea and kidney tissues after infection in ovo by avian infectious bronchitis coronavirus

Background: avian infectious bronchitis (ib) is one of the most serious diseases of economic importance in chickens; it is caused by the avian infectious coronavirus (ibv). information remains limited about the comparative protein expression profiles of chicken embryonic tissues in response to ibv infection in ovo. in this study,we analyzed the changes of protein expression in trachea and kidney tissues from chicken embryos,following ibv infection in ovo,using two-dimensional gel electrophoresis (2-de) coupled with matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry (maldi-tof-tof ms).results: 17 differentially expressed proteins from tracheal tissues and 19 differentially expressed proteins from kidney tissues were identified. these proteins mostly related to the cytoskeleton,binding of calcium ions,the stress response,anti-oxidative,and macromolecular metabolism. some of these altered proteins were confirmed further at the mrna level using real-time rt-pcr. moreover,western blotting analysis further confirmed the changes of annexin a5 and hspb1 during ibv infection.conclusions: to the best of our knowledge,we have performed the first analysis of the proteomic changes in chicken embryonic trachea and kidney tissues during ibv infection in ovo. the data obtained should facilitate a better understanding of the pathogenesis of ibv infection. © 2011 cao et al; licensee biomed central ltd.