Characterization of proteome alterations in Phanerochaete chrysosporium in response to lead exposure

Background: total soluble proteome alterations of white rot fungus phanerochaete chrysosporium in response to different doses (25,50 and 100 μm) of pb (ii) were characterized by 2de in combination with maldi-tof-ms.results: dose-dependent molecular response to pb (ii) involved a total of 14 up-regulated and 21 down-regulated proteins. the induction of an isoform of glyceraldehyde 3-phosphate dehydrogenase,alcohol dehydrogenase class v,mrna splicing factor,atp-dependent rna helicase,thioredoxin reductase and actin required a pb (ii) dose of at least 50 μm. analysis of the proteome dynamics of mid-exponential phase cells of p. chrysosporium subjected to 50 μm lead at exposure time intervals of 1,2,4 and 8 h,identified a total of 23 proteins in increased and 67 proteins in decreased amount. overall,the newly induced/strongly up-regulated proteins involved in (i) amelioration of lipid peroxidation products,(ii) defense against oxidative damage and redox metabolism,(iii) transcription,recombination and dna repair (iv) a yet unknown function represented by a putative protein.conclusion: the present study implicated the particular role of the elements of dna repair,post-tanscriptional regulation and heterotrimeric g protein signaling in response to pb (ii) stress as shown for the first time for a basidiomycete. © 2011 yi{dotless}ldi{dotless}ri{dotless}m et al; licensee biomed central ltd.