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Discrimination of grade 2 and 3 cervical intraepithelial neoplasia by means of analysis of water soluble proteins recovered from cervical biopsies
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نویسنده
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uleberg k.-e. ,munk a.c. ,brede c. ,gudlaugsson e. ,van diermen b. ,skaland i. ,malpica a. ,janssen e.a.m. ,hjelle a. ,baak j.p.a.
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منبع
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proteome science - 2011 - دوره : 9 - شماره : 0
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چکیده
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Background: cervical intraepithelial neoplasia (cin) grades 2 and 3 are usually grouped and treated in the same way as high grade
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کلیدواژه
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in spite of their different risk to cancer progression and spontaneous regression rates. CIN2-3 is usually diagnosed in formaldehyde-fixed paraffin embedded (FFPE) punch biopsies. This procedure virtually eliminates the availability of water-soluble proteins which could have diagnostic and prognostic value.Aim: To investigate whether a water-soluble protein-saving biopsy processing method followed by a proteomic analysis of supernatant samples using LC-MS/MS (LTQ Orbitrap) can be used to distinguish between CIN2 and CIN3.Methods: Fresh cervical punch biopsies from 20 women were incubated in RPMI1640 medium for 24 hours at 4°C for protein extraction and subsequently subjected to standard FFPE processing. P16 and Ki67-supported histologic consensus review CIN grade (CIN2
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آدرس
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pathology department,stavanger university hospital,armauer hansen road 20,stavanger,norway,the gade institute,university of bergen,bergen,norway,international research institute of stavanger (iris),stavanger, Norway, pathology department,stavanger university hospital,armauer hansen road 20,stavanger,norway,gynaecology department,stavanger university hospital,armauer hansen road 20,stavanger, Norway, department of medical biochemistry,stavanger university hospital,armauer hansen road 20,stavanger, Norway, pathology department,stavanger university hospital,armauer hansen road 20,stavanger,norway,international research institute of stavanger (iris),stavanger, Norway, pathology department,stavanger university hospital,armauer hansen road 20,stavanger, Norway, pathology department,stavanger university hospital,armauer hansen road 20,stavanger, Norway, departments of pathology and gynecologic oncology,the university of texas m. d. anderson cancer center,houston,tx, United States, pathology department,stavanger university hospital,armauer hansen road 20,stavanger, Norway, international research institute of stavanger (iris),stavanger, Norway, pathology department,stavanger university hospital,armauer hansen road 20,stavanger,norway,the gade institute,university of bergen,bergen, Norway
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Authors
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