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Proteomic analysis of pregnancy-related proteins from pig uterus endometrium during pregnancy
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نویسنده
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chae j.-i. ,kim j. ,lee s.g. ,jeon y.-j. ,kim d.-w. ,soh y. ,seo k.s. ,lee h.k. ,choi n.-j. ,ryu j. ,kang s. ,cho s.-k. ,lee d.-s. ,chung h.m. ,koo a.d.
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منبع
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proteome science - 2011 - دوره : 9 - شماره : 0
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چکیده
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Many important molecular events associated with implantation and development occur within the female reproductive tract,especially within the uterus endometrium,during pregnancy periods. the endometrium includes the mucosal lining of the uterus,which provides a suitable site for implantation and development of a fertilized egg and fetus. to date,the molecular cascades in the uterus endometrium during pregnancy periods in pigs have not been elucidated fully. in this study,we compared the functional regulated proteins in the endometrium during pregnancy periods with those in non-pregnant conditions and investigated changes in expression patterns during pregnancy (days 40,70,and 93) using two-dimensional gel electrophoresis (2-de) and western blotting. the functional regulated proteins were identified and discovered from differentially expressed proteins in the uterus endometrium during pregnancy. we discovered 820 protein spots in a proteomic analysis of uterus endometrium tissues with 2-de gels. we identified 63 of the 98 proteins regulated differentially among non-pregnant and pregnant tissues (matched and unmatched spots). interestingly,10 of these 63 proteins are development-,cytoskeleton- and chaperon-related proteins such as transferrin,protein dj-1,transgelin,galectin-1,septin 2,stathmin 1,cofilin 1,fascin 1,heat shock protein (hsp) 90β and hsp 27. the specific expression patterns of these proteins in the endometrium during pregnancy were confirmed by western blotting. our results suggest that the expressions of these genes involved in endometrium function and endometrium development from early to late gestation are associated with the regulation of endometrium development for maintaining pregnancy.© 2011 chae et al; licensee biomed central ltd.
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آدرس
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department of oral pharmacology,school of dentistry and institute of dental bioscience,bk21 project,chonbuk national university,jeonju (651-756), South Korea, graduate school of life science,cha stem cell institute,college of medicine,cha university,605-21 yeoksam 1 dong,gangnam gu,seoul 135-907, South Korea, department of obstetrics and gynecology,college of medicine,yeungnam university,daegu 705-717, South Korea, department of oral pharmacology,school of dentistry and institute of dental bioscience,bk21 project,chonbuk national university,jeonju (651-756), South Korea, department of oral pharmacology,school of dentistry and institute of dental bioscience,bk21 project,chonbuk national university,jeonju (651-756), South Korea, department of oral pharmacology,school of dentistry and institute of dental bioscience,bk21 project,chonbuk national university,jeonju (651-756), South Korea, department of animal science and technology,sunchon national university,suncheon 540-742, South Korea, genomic informatics center,hankyong national university,67 sukjong-dong,ansung-city,kyongi-do,456-749, South Korea, department of animal science,college of agricultural and life science,chonbuk national university,jeonju, South Korea, medical proteomics research center,kribb,daejeon, South Korea, medical proteomics research center,kribb,daejeon, South Korea, depart. of animal science,college of national resources and life science,pusan national university,miryang-si,gyeongnam 627-706, South Korea, college of natural sciences,kyungpook national university,daegu 702-701, South Korea, cha bio and diostech co.,ltd.,606-16 yeoksam 1 dong,gangnam gu,seoul 135-907,south korea,graduate school of life science,cha stem cell institute,college of medicine,cha university,605-21 yeoksam 1 dong,gangnam gu,seoul 135-907, South Korea, department of biotechnology,college of engineering daegu university,15 jillyang gyeongsan,gyeongbuk 712-714, South Korea
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Authors
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