Proteomics study of changes in soybean lines resistant and sensitive to Phytophthora sojae

Background: phytophthora sojae causes soybean root and stem rot,resulting in an annual loss of 1-2 billion us dollars in soybean production worldwide. a proteomic technique was used to determine the effects on soybean hypocotyls of infection with p. sojae.results: in the present study,46 differentially expressed proteins were identified in soybean hypocotyls infected with p. sojae,using two-dimensional electrophoresis and matrix-assisted laser desorption/ionization tandem time of flight (maldi-tof/tof). the expression levels of 26 proteins were significantly affected at various time points in the tolerant soybean line,yudou25,(12 up-regulated and 14 down-regulated). in contrast,in the sensitive soybean line,ng6255,only 20 proteins were significantly affected (11 up-regulated and 9 down-regulated). among these proteins,26% were related to energy regulation,15% to protein destination and storage,11% to defense against disease,11% to metabolism,9% to protein synthesis,4% to secondary metabolism,and 24% were of unknown function.conclusion: our study provides important information on the use of proteomic methods for studying protein regulation during plant-oomycete interactions. © 2011 zhang et al; licensee biomed central ltd.