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Improved protein arrays for quantitative systems analysis of the dynamics of signaling pathway interactions
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نویسنده
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wang x. ,dong y. ,jiwani a.j. ,zou y. ,pastor j. ,kuro-o m. ,habib a.a. ,ruan m. ,boothman d.a. ,yang c.-r.
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منبع
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proteome science - 2011 - دوره : 9 - شماره : 0
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چکیده
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An improved version of quantitative protein array platform utilizing linear quantum dot signaling for systematically measuring protein levels and phosphorylation states is presented. the signals are amplified linearly by a confocal laser quantum dot scanner resulting in ~1000-fold more sensitivity than traditional western blots,but are not linear by the enzyme-based amplification. software is developed to facilitate the quantitative readouts of signaling network activities. kinetics of egfrviii mutant signaling was analyzed to quantify cross-talks between egfr and other signaling pathways. © 2011 wang et al; licensee biomed central ltd.
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کلیدواژه
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Analysis; Cross-talkimage; Glioma; Protein array; Quantitative analysis; quantum dot; Signaling pathway; Systems biology
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آدرس
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harold c. simmons comprehensive cancer center,university of texas southwestern medical center at dallas,dallas,tx 75390,united states,department of radiation oncology,university of texas southwestern medical center at dallas,dallas,tx 75390, United States, harold c. simmons comprehensive cancer center,university of texas southwestern medical center at dallas,dallas,tx 75390,united states,department of pharmacology,university of texas southwestern medical center at dallas,dallas,tx 75390, United States, harold c. simmons comprehensive cancer center,university of texas southwestern medical center at dallas,dallas,tx 75390,united states,department of neurology and neurotherapeutics,university of texas southwestern medical center,dallas,tx 75390,united states,north texas va healthcare system,dallas,tx 75216, United States, harold c. simmons comprehensive cancer center,university of texas southwestern medical center at dallas,dallas,tx 75390,united states,department of pharmacology,university of texas southwestern medical center at dallas,dallas,tx 75390, United States, department of pathology,university of texas southwestern medical center at dallas,dallas,tx 75390, United States, harold c. simmons comprehensive cancer center,university of texas southwestern medical center at dallas,dallas,tx 75390,united states,department of pathology,university of texas southwestern medical center at dallas,dallas,tx 75390, United States, harold c. simmons comprehensive cancer center,university of texas southwestern medical center at dallas,dallas,tx 75390,united states,department of neurology and neurotherapeutics,university of texas southwestern medical center,dallas,tx 75390,united states,north texas va healthcare system,dallas,tx 75216, United States, vigenetech,inc.,carlisle,ma 01741, United States, harold c. simmons comprehensive cancer center,university of texas southwestern medical center at dallas,dallas,tx 75390,united states,department of pharmacology,university of texas southwestern medical center at dallas,dallas,tx 75390, United States, harold c. simmons comprehensive cancer center,university of texas southwestern medical center at dallas,dallas,tx 75390,united states,department of radiation oncology,university of texas southwestern medical center at dallas,dallas,tx 75390, United States
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Authors
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