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Label-free quantitative proteomics of CD133-positive liver cancer stem cells
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نویسنده
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tsai s.-t. ,tsou c.-c. ,mao w.-y. ,chang w.-c. ,han h.-y. ,hsu w.-l. ,li c.-l. ,shen c.-n. ,chen c.-h.
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منبع
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proteome science - 2012 - دوره : 10 - شماره : 1
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چکیده
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Background: cd133-positive liver cancer stem cells,which are characterized by their resistance to conventional chemotherapy and their tumor initiation ability at limited dilutions,have been recognized as a critical target in liver cancer therapeutics. in the current work,we developed a label-free quantitative method to investigate the proteome of cd133-positive liver cancer stem cells for the purpose of identifying unique biomarkers that can be utilized for targeting liver cancer stem cells. label-free quantitation was performed in combination with id-based elution time alignment by linear regression quantitation (ideal-q) and maxquant.results: initially,ideal-q analysis revealed that 151 proteins were differentially expressed in the cd133-positive hepatoma cells when compared with cd133-negative cells. we then analyzed these 151 differentially expressed proteins by maxquant software and identified 10 significantly up-regulated proteins. the results were further validated by rt-pcr,western blot,flow cytometry or immunofluorescent staining which revealed that prominin-1,annexin a1,annexin a3,transgelin,creatine kinase b,vimentin,and epcam were indeed highly expressed in the cd133-positive hepatoma cells.conclusions: these findings confirmed that mass spectrometry-based label-free quantitative proteomics can be used to gain insights into liver cancer stem cells. © 2012 tsai et al.; licensee biomed central ltd.
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کلیدواژه
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Hepatocellular carcinoma; Label-free quantitation; LC-MS/MS; Liver cancer stem cells
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آدرس
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institute of biochemistry and molecular biology,national yang-ming university,taipei,taiwan,genomics research center,academia sinica,taipei, Taiwan, institute of information science,academia sinica,taipei, Taiwan, genomics research center,academia sinica,taipei, Taiwan, genomics research center,academia sinica,taipei, Taiwan, genomics research center,academia sinica,taipei, Taiwan, institute of information science,academia sinica,taipei, Taiwan, genomics research center,academia sinica,taipei,taiwan,institute of cellular and organismic biology,academia sinica,taipei, Taiwan, institute of biochemistry and molecular biology,national yang-ming university,taipei,taiwan,genomics research center,academia sinica,taipei, Taiwan, institute of biochemistry and molecular biology,national yang-ming university,taipei,taiwan,genomics research center,academia sinica,taipei,taiwan,department of chemistry,national taiwan university,taipei,taiwan,institute of atomic and molecular sciences,academia sinica,taipei, Taiwan
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Authors
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