|
|
|
|
Proteomic sample preparation for blast wound characterization
|
|
|
|
|
|
|
|
نویسنده
|
chromy b.a. ,eldridge a. ,forsberg j.a. ,brown t.s. ,kirkup b.c. ,elster e. ,luciw p.
|
|
منبع
|
proteome science - 2014 - دوره : 12 - شماره : 1
|
|
چکیده
|
Background: blast wounds often involve diverse tissue types and require substantial time and treatment for appropriate healing. some of these subsequent wounds become colonized with bacteria requiring a better understanding of how the host responds to these bacteria and what proteomic factors contribute wound healing outcome. in addition,using reliable and effective proteomic sample preparation procedures can lead to novel biomarkers for improved diagnosis and therapy.results: to address this need,suitable sample preparation for 2-d dige proteomic characterization of wound effluent and serum samples from combat-wounded patients was investigated. initial evaluation of crude effluent and serum proved the necessity of high abundant protein depletion. subsequently,both samples were successfully depleted using agilent multiple affinity removal system and showed greatly improved 2-d spot maps,comprising 1,800 and 1,200 protein spots,respectively.conclusion: high abundant protein removal was necessary for both wound effluent and serum. this is the first study to show a successful method for high abundant protein depletion from wound effluent which is compatible with downstream 2-d dige analysis. this development allows for improved biomarker discovery in wound effluent and serum samples. © 2014 chromy et al.; licensee biomed central ltd.
|
|
کلیدواژه
|
2-D DIGE; Biomarker discovery; Blast wound; High abundant protein removal; Proteomics; Serum; Wound effluent
|
|
آدرس
|
department of pathology and laboratory medicine,school of medicine,university of california at davis,davis,ca, United States, department of pathology and laboratory medicine,school of medicine,university of california at davis,davis,ca,united states,center for comparative medicine,university of california at davis,davis,ca, United States, regenerative medicine department,naval medical research center,silver spring,md,united states,department of orthopaedics,national military medical center,bethesda,md,united states,norman m. rich department of surgery and surgical critical care institute,uniformed services university of the health sciences,bethesda,md, United States, department of wound infections,walter reed army institute of research,silver spring,md, United States, department of wound infections,walter reed army institute of research,silver spring,md,united states,department of medicine,f edward hebert school of medicine,uniformed services university of the health sciences,bethesda,md, United States, department of wound infections,walter reed army institute of research,silver spring,md,united states,norman m. rich department of surgery and surgical critical care institute,uniformed services university of the health sciences,bethesda,md, United States, department of pathology and laboratory medicine,school of medicine,university of california at davis,davis,ca,united states,center for comparative medicine,university of california at davis,davis,ca, United States
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Authors
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|