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Quantitative proteomics analysis of early recurrence/metastasis of huge hepatocellular carcinoma following radical resection
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نویسنده
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huang x. ,zeng y. ,xing x. ,zeng j. ,gao y. ,cai z. ,xu b. ,liu x. ,huang a. ,liu j.
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منبع
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proteome science - 2014 - دوره : 12 - شماره : 1
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چکیده
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Background: hepatic resection is the preferred treatment for huge hepatocellular carcinoma (>10 cm in diameter; h-hcc). however,the patients with h-hcc suffer from poor prognosis due to the early recurrence/metastasis. the underlying mechanism of h-hcc's early recurrence/metastasis is currently not well understood.results: here,we describe an isobaric tags for relative and absolute quantification (itraq)-based quantitative proteomics approach to analyze the early recurrence/metastasis related proteins of h-hcc after radical resection through multidimensional chromatography coupled with tandem mass spectrometry (2dlc-ms/ms). the different protein expression profiles between the early recurrence/metastasis within 6 months(r/m≤6months) and late recurrence/metastasis within 6-12 months after surgery (r/m6-12months) were confirmed and might reveal different underlying molecular mechanisms. we identified 44 and 49 significantly differentially expressed proteins in the r/m≤6months group and the r/m6-12months group compared to the group who had no recurrence within 2 years post surgery (the nr/m group),respectively. moreover,among those proteins,s100a12 and amacr were down regulated in the r/m≤6months group but up-regulated in the r/m6-12months group; and this regulation was further confirmed in mrna and protein level by q-pcr,western-blot and immunohistochemistry (ihc).conclusions: this current study presents the first proteomic profile of the early recurrence/metastasis of h-hcc. the results suggest that s100a12 and amacr might be potential prognostic markers for predicting the early recurrence/metastasis of h-hcc after hepatectomy. © 2014 huang et al.; licensee biomed central ltd.
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کلیدواژه
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Early recurrence/metastasis; Huge hepatocellular carcinoma (H-HCC); iTRAQ; Potential prognostic biomarker; Quantitative proteomics
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آدرس
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mengchao hepatobiliary hospital of fujian medical university,350025 fuzhou,china,the liver center of fujian province,fujian medical university,350025 fuzhou, China, mengchao hepatobiliary hospital of fujian medical university,350025 fuzhou,china,the liver center of fujian province,fujian medical university,350025 fuzhou,china,liver disease center,the first affiliated hospital of fujian medical university,350005 fuzhou, China, mengchao hepatobiliary hospital of fujian medical university,350025 fuzhou,china,the liver center of fujian province,fujian medical university,350025 fuzhou, China, mengchao hepatobiliary hospital of fujian medical university,350025 fuzhou,china,the liver center of fujian province,fujian medical university,350025 fuzhou,china,liver disease center,the first affiliated hospital of fujian medical university,350005 fuzhou, China, mengchao hepatobiliary hospital of fujian medical university,350025 fuzhou,china,the liver center of fujian province,fujian medical university,350025 fuzhou, China, mengchao hepatobiliary hospital of fujian medical university,350025 fuzhou,china,the liver center of fujian province,fujian medical university,350025 fuzhou, China, mengchao hepatobiliary hospital of fujian medical university,350025 fuzhou,china,the liver center of fujian province,fujian medical university,350025 fuzhou,china,liver disease center,the first affiliated hospital of fujian medical university,350005 fuzhou, China, mengchao hepatobiliary hospital of fujian medical university,350025 fuzhou,china,the liver center of fujian province,fujian medical university,350025 fuzhou, China, mengchao hepatobiliary hospital of fujian medical university,350025 fuzhou,china,the liver center of fujian province,fujian medical university,350025 fuzhou,china,department of pathology,school of basic medical science,fujian medical university,350004 fuzhou, China, mengchao hepatobiliary hospital of fujian medical university,350025 fuzhou,china,the liver center of fujian province,fujian medical university,350025 fuzhou,china,liver disease center,the first affiliated hospital of fujian medical university,350005 fuzhou, China
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Authors
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