Proteomic study of sporadic inclusion body myositis

Background: sporadic inclusion body myositis (s-ibm) is the most commonly occurring acquired inflammatory myopathy in elderly people (>45 years); however,pathogenic mechanisms are poorly understood and diagnostic tools are limited. in view of this,new therapeutic and diagnostic molecular markers for s-ibm need to be identified.experimental design: in this study,the proteomes from three s-ibm cases were compared with those from three cases of neurogenic muscular atrophy (control). proteins were separated by 2-dimensional polyacrylamide gel electrophoresis and profiled by mass spectrometric sequencing and subsequently validated by western blot.results: differential expression was noted in 29 proteins (16 upregulated and 13 downregulated) in s-ibm compared with the control group. functions of these proteins include oxidative stress response,regulation of apoptosis,signal transduction,and cytoskeleton. expression of both amyloid precursor protein (app) and αb-crystallin was increased in s-ibm cases.conclusions: our study reveals a unique pattern of protein expression in s-ibm,which should be further investigated in a wider cohort of ibm patients to fully realize the potential diagnostic or therapeutic benefits. © 2014 li et al.; licensee biomed central ltd.