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Protective mAbs and Cross-Reactive mAbs Raised by Immunization with Engineered Marburg Virus GPs
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نویسنده
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fusco m.l. ,hashiguchi t. ,cassan r. ,biggins j.e. ,murin c.d. ,warfield k.l. ,li s. ,holtsberg f.w. ,shulenin s. ,vu h. ,olinger g.g. ,kim d.h. ,whaley k.j. ,zeitlin l. ,ward a.b. ,nykiforuk c. ,aman m.j. ,berry j. ,saphire e.o.
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منبع
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plos pathogens - 2015 - دوره : 11 - شماره : 6
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چکیده
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The filoviruses,which include the marburg- and ebolaviruses,have caused multiple outbreaks among humans this decade. antibodies against the filovirus surface glycoprotein (gp) have been shown to provide life-saving therapy in nonhuman primates,but such antibodies are generally virus-specific. many monoclonal antibodies (mabs) have been described against ebola virus. in contrast,relatively few have been described against marburg virus. here we present ten mabs elicited by immunization of mice using recombinant mucin-deleted gps from different marburg virus (marv) strains. surprisingly,two of the mabs raised against marv gp also cross-react with the mucin-deleted gp cores of all tested ebolaviruses (ebola,sudan,bundibugyo,reston),but these epitopes are masked differently by the mucin-like domains themselves. the most efficacious mabs in this panel were found to recognize a novel “wing” feature on the gp2 subunit that is unique to marburg and does not exist in ebola. two of these anti-wing antibodies confer 90 and 100% protection,respectively,one hour post-exposure in mice challenged with marv.
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آدرس
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department of immunology and microbial science,the scripps research institute,la jolla,ca, United States, department of immunology and microbial science,the scripps research institute,la jolla,ca,united states,department of virology,kyushu university,fukuoka, Japan, emergent biosolutions (formerly cangene corporation),winnipeg,mb, Canada, division of virology,united states army research institute for infectious disease,ft. detrickmd,united states,integrated biotherapeutics,inc.,gaithersburg,md, United States, department of immunology and microbial science,the scripps research institute,la jolla,ca,united states,department of integrative structural and computational biology,the scripps research institute,la jolla,california, United States, integrated biotherapeutics,inc.,gaithersburg,md, United States, department of medicine,university of california san diego,la jolla,ca, United States, integrated biotherapeutics,inc.,gaithersburg,md, United States, integrated biotherapeutics,inc.,gaithersburg,md, United States, integrated biotherapeutics,inc.,gaithersburg,md, United States, division of virology,united states army research institute for infectious disease,ft. detrickmd,united states,integrated research facility,niaid,national institutes of health,frederick,md, United States, mapp biopharmaceutical,inc.,san diego,ca, United States, mapp biopharmaceutical,inc.,san diego,ca, United States, mapp biopharmaceutical,inc.,san diego,ca, United States, department of integrative structural and computational biology,the scripps research institute,la jolla,california, United States, emergent biosolutions (formerly cangene corporation),winnipeg,mb, Canada, integrated biotherapeutics,inc.,gaithersburg,md, United States, emergent biosolutions (formerly cangene corporation),winnipeg,mb, Canada, department of immunology and microbial science,the scripps research institute,la jolla,ca,united states,skaggs institute for chemical biology,the scripps research institute,la jolla,ca, United States
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Authors
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