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The Fungal Exopolysaccharide Galactosaminogalactan Mediates Virulence by Enhancing Resistance to Neutrophil Extracellular Traps
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نویسنده
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lee m.j. ,liu h. ,barker b.m. ,snarr b.d. ,gravelat f.n. ,al abdallah q. ,gavino c. ,baistrocchi s.r. ,ostapska h. ,xiao t. ,ralph b. ,solis n.v. ,lehoux m. ,baptista s.d. ,thammahong a. ,cerone r.p. ,kaminskyj s.g.w. ,guiot m.-c. ,latgé j.-p. ,fontaine t. ,vinh d.c. ,filler s.g. ,sheppard d.c.
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منبع
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plos pathogens - 2015 - دوره : 11 - شماره : 10
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چکیده
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Of the over 250 aspergillus species,aspergillus fumigatus accounts for up to 80% of invasive human infections. a. fumigatus produces galactosaminogalactan (gag),an exopolysaccharide composed of galactose and n-acetyl-galactosamine (galnac) that mediates adherence and is required for full virulence. less pathogenic aspergillus species were found to produce gag with a lower galnac content than a. fumigatus and expressed minimal amounts of cell wall-bound gag. increasing the galnac content of gag of the minimally pathogenic a. nidulans,either through overexpression of the a. nidulans epimerase ugeb or by heterologous expression of the a. fumigatus epimerase uge3 increased the amount of cell wall bound gag,augmented adherence in vitro and enhanced virulence in corticosteroid-treated mice to levels similar to a. fumigatus. the enhanced virulence of the overexpression strain of a. nidulans was associated with increased resistance to nadph oxidase-dependent neutrophil extracellular traps (nets) in vitro,and was not observed in neutropenic mice or mice deficient in nadph-oxidase that are unable to form nets. collectively,these data suggest that cell wall-bound gag enhances virulence through mediating resistance to nets. © 2015 lee et al.
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آدرس
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department of microbiology & immunology,mcgill university,montreal,qc,canada,department of pathology & laboratory medicine,david geffen school of medicine at university of california,los angeles,los angeles,ca, United States, division of infectious diseases,la biomedical research institute at harbor—ucla,torrance,ca, United States, department of immunology and infectious diseases,montana state university,bozeman,mt,united states,center for fungal pathogens,tgen-north,flagstaff,az, United States, department of microbiology & immunology,mcgill university,montreal,qc, Canada, department of microbiology & immunology,mcgill university,montreal,qc, Canada, department of microbiology & immunology,mcgill university,montreal,qc,canada,department of microbiology & immunology,university of south alabama,mobile,al, United States, infectious disease susceptibility program,mcgill university health centre,montreal,qc, Canada, department of microbiology & immunology,mcgill university,montreal,qc, Canada, department of microbiology & immunology,mcgill university,montreal,qc, Canada, department of microbiology & immunology,mcgill university,montreal,qc, Canada, department of microbiology & immunology,mcgill university,montreal,qc, Canada, division of infectious diseases,la biomedical research institute at harbor—ucla,torrance,ca, United States, department of microbiology & immunology,mcgill university,montreal,qc, Canada, department of microbiology & immunology,mcgill university,montreal,qc, Canada, department of microbiology & immunology,geisel school of medicine at dartmouth,hanover,nh, United States, department of microbiology & immunology,mcgill university,montreal,qc, Canada, department of biology university of saskatchewan,saskatoon,sk, Canada, department of pathology montreal neurological hospital,montreal,qc, Canada, aspergillus unit,institut pasteur,paris, France, aspergillus unit,institut pasteur,paris, France, infectious disease susceptibility program,mcgill university health centre,montreal,qc, Canada, division of infectious diseases,la biomedical research institute at harbor—ucla,torrance,ca,united states,david geffen school of medicine,university of california,los angeles,los angeles,ca, United States, department of microbiology & immunology,mcgill university,montreal,qc,canada,department of medicine,mcgill university,montreal,qc, Canada
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Authors
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