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Phospho-dependent Regulation of SAMHD1 Oligomerisation Couples Catalysis and Restriction
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نویسنده
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arnold l.h. ,groom h.c.t. ,kunzelmann s. ,schwefel d. ,caswell s.j. ,ordonez p. ,mann m.c. ,rueschenbaum s. ,goldstone d.c. ,pennell s. ,howell s.a. ,stoye j.p. ,webb m. ,taylor i.a. ,bishop k.n.
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منبع
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plos pathogens - 2015 - دوره : 11 - شماره : 10
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چکیده
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Samhd1 restricts hiv-1 infection of myeloid-lineage and resting cd4+ t-cells. most likely this occurs through deoxynucleoside triphosphate triphosphohydrolase activity that reduces cellular dntp to a level where reverse transcriptase cannot function,although alternative mechanisms have been proposed recently. here,we present combined structural and virological data demonstrating that in addition to allosteric activation and triphosphohydrolase activity,restriction correlates with the capacity of samhd1 to form “long-lived” enzymatically competent tetramers. tetramer disruption invariably abolishes restriction but has varied effects on in vitro triphosphohydrolase activity. samhd1 phosphorylation also ablates restriction and tetramer formation but without affecting triphosphohydrolase steady-state kinetics. however phospho-samhd1 is unable to catalyse dntp turnover under conditions of nucleotide depletion. based on our findings we propose a model for phosphorylation-dependent regulation of samhd1 activity where dephosphorylation switches housekeeping samhd1 found in cycling cells to a high-activity stable tetrameric form that depletes and maintains low levels of dntps in differentiated cells. © 2015 arnold et al.
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آدرس
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the francis crick institute,mill hill laboratory,the ridgeway,mill hill,london, United Kingdom, the francis crick institute,mill hill laboratory,the ridgeway,mill hill,london, United Kingdom, the francis crick institute,mill hill laboratory,the ridgeway,mill hill,london, United Kingdom, the francis crick institute,mill hill laboratory,the ridgeway,mill hill,london, United Kingdom, the francis crick institute,mill hill laboratory,the ridgeway,mill hill,london, United Kingdom, the francis crick institute,mill hill laboratory,the ridgeway,mill hill,london, United Kingdom, the francis crick institute,mill hill laboratory,the ridgeway,mill hill,london, United Kingdom, the francis crick institute,mill hill laboratory,the ridgeway,mill hill,london, United Kingdom, the francis crick institute,mill hill laboratory,the ridgeway,mill hill,london,united kingdom,school of biological sciences,university of auckland,auckland, New Zealand, the francis crick institute,mill hill laboratory,the ridgeway,mill hill,london, United Kingdom, the francis crick institute,mill hill laboratory,the ridgeway,mill hill,london, United Kingdom, the francis crick institute,mill hill laboratory,the ridgeway,mill hill,london,united kingdom,imperial college london,london, United Kingdom, centre for genomic medicine,institute for human development,university of manchester,manchester, United Kingdom, the francis crick institute,mill hill laboratory,the ridgeway,mill hill,london, United Kingdom, the francis crick institute,mill hill laboratory,the ridgeway,mill hill,london, United Kingdom
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Authors
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