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IFNs Modify the Proteome of Legionella-Containing Vacuoles and Restrict Infection Via IRG1-Derived Itaconic Acid
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نویسنده
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naujoks j. ,tabeling c. ,dill b.d. ,hoffmann c. ,brown a.s. ,kunze m. ,kempa s. ,peter a. ,mollenkopf h.-j. ,dorhoi a. ,kershaw o. ,gruber a.d. ,sander l.e. ,witzenrath m. ,herold s. ,nerlich a. ,hocke a.c. ,van driel i. ,suttorp n. ,bedoui s. ,hilbi h. ,trost m. ,opitz b.
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منبع
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plos pathogens - 2016 - دوره : 12 - شماره : 2
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چکیده
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Macrophages can be niches for bacterial pathogens or antibacterial effector cells depending on the pathogen and signals from the immune system. here we show that type i and ii ifns are master regulators of gene expression during legionella pneumophila infection,and activators of an alveolar macrophage-intrinsic immune response that restricts bacterial growth during pneumonia. quantitative mass spectrometry revealed that both ifns substantially modify legionella-containing vacuoles,and comparative analyses reveal distinct subsets of transcriptionally and spatially ifn-regulated proteins. immune-responsive gene (irg)1 is induced by ifns in mitochondria that closely associate with legionella-containing vacuoles,and mediates production of itaconic acid. this metabolite is bactericidal against intravacuolar l. pneumophila as well as extracellular multidrug-resistant gram-positive and -negative bacteria. our study explores the overall role ifns play in inducing substantial remodeling of bacterial vacuoles and in stimulating production of irg1-derived itaconic acid which targets intravacuolar pathogens. irg1 or its product itaconic acid might be therapeutically targetable to fight intracellular and drug-resistant bacteria. © 2016 naujoks et al.
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آدرس
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department of internal medicine/infectious diseases and pulmonary medicine,charité university medicine berlin,berlin, Germany, department of internal medicine/infectious diseases and pulmonary medicine,charité university medicine berlin,berlin, Germany, mrc protein phosphorylation unit,university of dundee,dundee,united kingdom,proteomics resource center,the rockefeller university,new york,ny, United States, max-von-pettenkofer institute,ludwig maximilian university,munich, Germany, the department of biochemistry and molecular biology,the university of melbourne,melbourne, Australia, department of internal medicine/infectious diseases and pulmonary medicine,charité university medicine berlin,berlin, Germany, integrative metabolomics and proteomics,institute of medical systems biology/max-delbrueck center for molecular medicine,berlin, Germany, department of internal medicine/infectious diseases and pulmonary medicine,charité university medicine berlin,berlin, Germany, max planck institute for infection biology,berlin, Germany, max planck institute for infection biology,berlin, Germany, department of veterinary pathology,free university berlin,berlin, Germany, department of veterinary pathology,free university berlin,berlin, Germany, department of internal medicine/infectious diseases and pulmonary medicine,charité university medicine berlin,berlin, Germany, department of internal medicine/infectious diseases and pulmonary medicine,charité university medicine berlin,berlin, Germany, medizinische klinik ii,university giessen and marburg lung center,justus-liebig-university giessen,giessen, Germany, department of internal medicine/infectious diseases and pulmonary medicine,charité university medicine berlin,berlin, Germany, department of internal medicine/infectious diseases and pulmonary medicine,charité university medicine berlin,berlin, Germany, the department of biochemistry and molecular biology,the university of melbourne,melbourne, Australia, department of internal medicine/infectious diseases and pulmonary medicine,charité university medicine berlin,berlin, Germany, the department of microbiology and immunology,the university of melbourne,melbourne, Australia, max-von-pettenkofer institute,ludwig maximilian university,munich,germany,institute of medical microbiology,university of zurich,zurich, Switzerland, mrc protein phosphorylation unit,university of dundee,dundee, United Kingdom, department of internal medicine/infectious diseases and pulmonary medicine,charité university medicine berlin,berlin, Germany
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Authors
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