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High-Resolution Crystal Structures Elucidate the Molecular Basis of Cholera Blood Group Dependence
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نویسنده
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heggelund j.e. ,burschowsky d. ,bjørnestad v.a. ,hodnik v. ,anderluh g. ,krengel u.
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منبع
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plos pathogens - 2016 - دوره : 12 - شماره : 4
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چکیده
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Cholera is the prime example of blood-group-dependent diseases,with individuals of blood group o experiencing the most severe symptoms. the cholera toxin is the main suspect to cause this relationship. we report the high-resolution crystal structures (1.1–1.6 å) of the native cholera toxin b-pentamer for both classical and el tor biotypes,in complexes with relevant blood group determinants and a fragment of its primary receptor,the gm1 ganglioside. the blood group a determinant binds in the opposite orientation compared to previously published structures of the cholera toxin,whereas the blood group h determinant,characteristic of blood group o,binds in both orientations. h-determinants bind with higher affinity than a-determinants,as shown by surface plasmon resonance. together,these findings suggest why blood group o is a risk factor for severe cholera. © 2016 heggelund et al.
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آدرس
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department of chemistry,university of oslo,blindern, Norway, department of chemistry,university of oslo,blindern, Norway, department of chemistry,university of oslo,blindern, Norway, department of biology,biotechnical faculty,university of ljubljana,ljubljana, Slovenia, department of biology,biotechnical faculty,university of ljubljana,ljubljana,slovenia,the national institute of chemistry,ljubljana, Slovenia, department of chemistry,university of oslo,blindern, Norway
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Authors
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