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Differences in the Selection Bottleneck between Modes of Sexual Transmission Influence the Genetic Composition of the HIV-1 Founder Virus
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نویسنده
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tully d.c. ,ogilvie c.b. ,batorsky r.e. ,bean d.j. ,power k.a. ,ghebremichael m. ,bedard h.e. ,gladden a.d. ,seese a.m. ,amero m.a. ,lane k. ,mcgrath g. ,bazner s.b. ,tinsley j. ,lennon n.j. ,henn m.r. ,brumme z.l. ,norris p.j. ,rosenberg e.s. ,mayer k.h. ,jessen h. ,kosakovsky pond s.l. ,walker b.d. ,altfeld m. ,carlson j.m. ,allen t.m.
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منبع
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plos pathogens - 2016 - دوره : 12 - شماره : 5
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چکیده
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Due to the stringent population bottleneck that occurs during sexual hiv-1 transmission,systemic infection is typically established by a limited number of founder viruses. elucidation of the precise forces influencing the selection of founder viruses may reveal key vulnerabilities that could aid in the development of a vaccine or other clinical interventions. here,we utilize deep sequencing data and apply a genetic distance-based method to investigate whether the mode of sexual transmission shapes the nascent founder viral genome. analysis of 74 acute and early hiv-1 infected subjects revealed that 83% of men who have sex with men (msm) exhibit a single founder virus,levels similar to those previously observed in heterosexual (hsx) transmission. in a metadata analysis of a total of 354 subjects,including hsx,msm and injecting drug users (idu),we also observed no significant differences in the frequency of single founder virus infections between hsx and msm transmissions. however,comparison of hiv-1 envelope sequences revealed that hsx founder viruses exhibited a greater number of codon sites under positive selection,as well as stronger transmission indices possibly reflective of higher fitness variants. moreover,specific genetic “signatures” within msm and hsx founder viruses were identified,with single polymorphisms within gp41 enriched among hsx viruses while more complex patterns,including clustered polymorphisms surrounding the cd4 binding site,were enriched in msm viruses. while our findings do not support an influence of the mode of sexual transmission on the number of founder viruses,they do demonstrate that there are marked differences in the selection bottleneck that can significantly shape their genetic composition. this study illustrates the complex dynamics of the transmission bottleneck and reveals that distinct genetic bottleneck processes exist dependent upon the mode of hiv-1 transmission. © 2016 tully et al.
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آدرس
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ragon institute of mgh,mit and harvard,cambridge,ma, United States, ragon institute of mgh,mit and harvard,cambridge,ma, United States, ragon institute of mgh,mit and harvard,cambridge,ma, United States, ragon institute of mgh,mit and harvard,cambridge,ma, United States, ragon institute of mgh,mit and harvard,cambridge,ma, United States, ragon institute of mgh,mit and harvard,cambridge,ma, United States, ragon institute of mgh,mit and harvard,cambridge,ma, United States, ragon institute of mgh,mit and harvard,cambridge,ma, United States, ragon institute of mgh,mit and harvard,cambridge,ma, United States, ragon institute of mgh,mit and harvard,cambridge,ma, United States, ragon institute of mgh,mit and harvard,cambridge,ma, United States, division of infectious disease,massachusetts general hospital,boston,ma, United States, division of infectious disease,massachusetts general hospital,boston,ma, United States, the fenway institute,fenway health,boston,ma, United States, broad institute of mit and harvard,cambridge,ma, United States, broad institute of mit and harvard,cambridge,ma, United States, faculty of health sciences,simon fraser university,vancouver,bc, Canada, blood systems research institute,san francisco,ca, United States, division of infectious disease,massachusetts general hospital,boston,ma, United States, the fenway institute,fenway health,boston,ma, United States, hiv clinic praxis,jessen,berlin, Germany, department of medicine,university of california san diego,la jolla,ca, United States, ragon institute of mgh,mit and harvard,cambridge,ma,united states,howard hughes medical institute,chevy chase,md, United States, ragon institute of mgh,mit and harvard,cambridge,ma,united states,heinrich-pette-institut,hamburg, Germany, microsoft research,redmond,wa, United States, ragon institute of mgh,mit and harvard,cambridge,ma, United States
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Authors
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