Mosquito Saliva Increases Endothelial Permeability in the Skin,Immune Cell Migration,and Dengue Pathogenesis during Antibody-Dependent Enhancement

Dengue remains the most prevalent arthropod-borne viral disease in humans. while probing for blood vessels,aedes aegypti and ae. albopictus mosquitoes transmit the four serotypes of dengue virus (denv1-4) by injecting virus-containing saliva into the skin. even though arthropod saliva is known to facilitate transmission and modulate host responses to other pathogens,the full impact of mosquito saliva on dengue pathogenesis is still not well understood. inoculating mice lacking the interferon-α/β receptor intradermally with denv revealed that mosquito salivary gland extract (sge) exacerbates dengue pathogenesis specifically in the presence of enhancing serotype-cross-reactive antibodies—when individuals already carry an increased risk for severe disease. we further establish that sge increases viral titers in the skin,boosts antibody-enhanced denv infection of dendritic cells and macrophages in the dermis,and amplifies dendritic cell migration to skin-draining lymph nodes. we demonstrate that sge directly disrupts endothelial barrier function in vitro and induces endothelial permeability in vivo in the skin. finally,we show that surgically removing the site of denv transmission in the skin after 4 hours rescued mice from disease in the absence of sge,but no longer prevented lethal antibody-enhanced disease when sge was present. these results indicate that sge accelerates the dynamics of dengue pathogenesis after virus transmission in the skin and induces severe antibody-enhanced disease systemically. our study reveals novel aspects of dengue pathogenesis and suggests that animal models of dengue and pre-clinical testing of dengue vaccines should consider mosquito-derived factors as well as enhancing antibodies. © 2016 schmid et al.