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Apicoplast-Localized Lysophosphatidic Acid Precursor Assembly Is Required for Bulk Phospholipid Synthesis in Toxoplasma gondii and Relies on an Algal/Plant-Like Glycerol 3-Phosphate Acyltransferase
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نویسنده
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amiar s. ,macrae j.i. ,callahan d.l. ,dubois d. ,van dooren g.g. ,shears m.j. ,cesbron-delauw m.-f. ,maréchal e. ,mcconville m.j. ,mcfadden g.i. ,yamaryo-botté y. ,botté c.y.
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منبع
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plos pathogens - 2016 - دوره : 12 - شماره : 8
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چکیده
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Most apicomplexan parasites possess a non-photosynthetic plastid (the apicoplast),which harbors enzymes for a number of metabolic pathways,including a prokaryotic type ii fatty acid synthesis (fasii) pathway. in toxoplasma gondii,the causative agent of toxoplasmosis,the fasii pathway is essential for parasite growth and infectivity. however,little is known about the fate of fatty acids synthesized by fasii. in this study,we have investigated the function of a plant-like glycerol 3-phosphate acyltransferase (tgats1) that localizes to the t. gondii apicoplast. knock-down of tgats1 resulted in significantly reduced incorporation of fasii-synthesized fatty acids into phosphatidic acid and downstream phospholipids and a severe defect in intracellular parasite replication and survival. lipidomic analysis demonstrated that lipid precursors are made in,and exported from,the apicoplast for de novo biosynthesis of bulk phospholipids. this study reveals that the apicoplast-located fasii and ats1,which are primarily used to generate plastid galactolipids in plants and algae,instead generate bulk phospholipids for membrane biogenesis in t. gondii. © 2016 amiar et al.
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آدرس
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apicolipid group,institute for advanced biosciences umr5309,cnrs,université grenoble alpes,inserm,grenoble, France, the francis crick institute,the ridgeway,mill hill,london,united kingdom,department of biochemistry and molecular biology,bio21 molecular science & biotechnology institute,university of melbourne,melbourne,vic, Australia, centre for chemistry and biotechnology,school of life and environmental sciences,deakin university,burwood,vic,australia,school of biosciences,university of melbourne,melbourne,vic, Australia, apicolipid group,institute for advanced biosciences umr5309,cnrs,université grenoble alpes,inserm,grenoble, France, research school of biology,australian national university,canberra,act, Australia, apicolipid group,institute for advanced biosciences umr5309,cnrs,université grenoble alpes,inserm,grenoble,france,school of biosciences,university of melbourne,melbourne,vic, Australia, bni group,timc-imag umr5525 cnrs,université grenoble alpes,grenoble, France, unité de recherche (umr) 5168,cnrs,cea,inra,université grenoble alpes,grenoble, France, department of biochemistry and molecular biology,bio21 molecular science & biotechnology institute,university of melbourne,melbourne,vic, Australia, school of biosciences,university of melbourne,melbourne,vic, Australia, apicolipid group,institute for advanced biosciences umr5309,cnrs,université grenoble alpes,inserm,grenoble, France, apicolipid group,institute for advanced biosciences umr5309,cnrs,université grenoble alpes,inserm,grenoble, France
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Authors
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