Correction to: Spliceosome SNRNP200 Promotes Viral RNA Sensing and IRF3 Activation of Antiviral Response (PLOS Pathogens,(2016),12,7,(e1005772),10.1371/journal.ppat.1005772)

The authors would like to correct figures 1,6,and 8,as changes were made to these figures in preparation for publication that were not indicated in the original figures and figure legends. in addition,some of those changes resulted in errors in the presentation of the final figures. in figure 1c cropping was done during image preparation,and a vertical line should have been inserted in the western blot presented to indicate the cropping. figure 1c was assembled from a larger experiment,which included a 72-hour time point infection,as shown in the un-cropped blot in s1 fig here. the corrected fig 1c has been modified to show the above mentioned modification. the raw blots for fig 1c are also presented here in s1 fig. (figure presented.) in figures 6a,6b,and 6c cropping was done during image preparation and vertical lines should have been inserted in the western blot presented to indicate the cropping. figures 6a,6b,and 6c were generated from a larger experiment as shown in the un-cropped blot in s2 fig. for the corrected fig 6a,the authors performed a new experiment as described in the original manuscript,which is now presented here. vertical lines have been inserted to the corrected fig 6b to show where cropping occurred. the corrected fig 6c was reorganized to show where the cropping occurred. the raw blots for fig 6a,6b and 6c are also presented here in s2 fig. (figure presented.) in figures 8a and 8b cropping was done during image preparation,which resulted in the duplication of an actin panel in figure 8b. figures 8a and 8b were generated from a larger experiment as shown in the un-cropped blot in s3 fig. for the corrected fig 8a and 8b,the authors have provided a new fig 8 that includes an a/b panel,which is made from the complete unspliced data. the raw blots for fig 8 are presented here in s3 fig. (figure presented.) the authors confirm that these changes do not alter their findings. the authors have provided raw,uncropped blots as supporting information. supporting information s1 fig uncropped blots of fig 1c. immunoblot analysis of hek 293t cells infected with sev for 8,24,48 or 72 hours following treatment with shnt or shsnrnp200 for three days. sev and ifit1 proteins are resolved by immunobloting at the indicated time. for clarity,the lane for the 72-hour time point infection was removed of fig 1c. red boxes indicate the cropped area used in fig 1c. the immunobloting of actin is not available as the raw data could not be retrieved. (tif) s2 fig uncropped blots of figs 6a,6b and 6c. (a) hek 293t cells are transfected with flag-eyfp (control),flag-snrnp200 or flag-snrnp200 s1087l mutant expressing plasmids for 48 hours. cell lysates are prepared following 16 hours of sev infection and subjected to immunoprecipitation with anti-flag antibodies. cell lysates and immune complexes are resolved by immunobloting analysis using anti-flag and anti-tbk1 antibodies. red boxes indicate the cropped area used in fig 6a. (b) immunoprecipitation of flag-snrnp200 c-terminal deletion mutants. hek 293t cells are transfected with flag-eyfp (control),flag-snrnp200 and flag-deletion mutants expressing plasmids for 48 hours. cell lysates are prepared following 16 hours of sev infection and subjected to immunoprecipitation with anti-flag antibodies. immune complexes are resolved by immunobloting analysis using anti-flag and anti-tbk1 antibodies. left. cropping was done on the blots to remove lanes of the larger experiment. right. cropping was done to enhance clarity and match the presentation of the overall figure. red boxes indicate the cropped area used in fig 6b. (c) immunoprecipitation of flag-snrnp200 sec63-1,hb-hlh or hlh-ig subdomains of sec63-1 and sec63-2 are performed and analyzed as indicated in (a). cropping was done on the blots from a larger experiment to remove unnecessary lanes and enhance clarity. red boxes indicate the cropped area used in fig 6c. (tif) s3 fig uncropped blots of fig 8a-b. (a-b) mdm are transfected with a pool of sirna targeting snrnp200 for 48 hours and infected with sev. at 1,3 and 5 hours post-infection,cells are harvested and selected proteins (snrnp200,ifit1,ifih1,irf3,irf3-p386,sev and actin) are resolved by immunobloting of cells lysates and compared to control cells treated with scrambled sirna. red boxes indicate the cropped area used in fig 8a-b. © 2017 tremblay et al.