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The effective rate of influenza reassortment is limited during human infection
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نویسنده
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sobel leonard a. ,mcclain m.t. ,smith g.j.d. ,wentworth d.e. ,halpin r.a. ,lin x. ,ransier a. ,stockwell t.b. ,das s.r. ,gilbert a.s. ,lambkin-williams r. ,ginsburg g.s. ,woods c.w. ,koelle k. ,illingworth c.j.r.
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منبع
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plos pathogens - 2017 - دوره : 13 - شماره : 2
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چکیده
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We characterise the evolutionary dynamics of influenza infection described by viral sequence data collected from two challenge studies conducted in human hosts. viral sequence data were collected at regular intervals from infected hosts. changes in the sequence data observed across time show that the within-host evolution of the virus was driven by the reversion of variants acquired during previous passaging of the virus. treatment of some patients with oseltamivir on the first day of infection did not lead to the emergence of drug resistance variants in patients. using an evolutionary model,we inferred the effective rate of reassortment between viral segments,measuring the extent to which randomly chosen viruses within the host exchange genetic material. we find strong evidence that the rate of effective reassortment is low,such that genetic associations between polymorphic loci in different segments are preserved during the course of an infection in a manner not compatible with epistasis. combining our evidence with that of previous studies we suggest that spatial heterogeneity in the viral population may reduce the extent to which reassortment is observed. our results do not contradict previous findings of high rates of viral reassortment in vitro and in small animal studies,but indicate that in human hosts the effective rate of reassortment may be substantially more limited. © 2017 sobel leonard et al.
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آدرس
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department of biology,duke university,durham,nc, United States, duke center for applied genomics and precision medicine,durham,nc, United States, programme in emerging infectious diseases,duke-nus medical school, Singapore, j. craig venter institute,rockville,md,united states,centers for disease control and prevention,ms-g16,atlanta,ga, United States, j. craig venter institute,rockville,md, United States, j. craig venter institute,rockville,md, United States, j. craig venter institute,rockville,md, United States, j. craig venter institute,rockville,md, United States, j. craig venter institute,rockville,md, United States, hvivo plc,the qmb innovation centre,queen mary,university of london,london, United Kingdom, hvivo plc,the qmb innovation centre,queen mary,university of london,london, United Kingdom, duke center for applied genomics and precision medicine,durham,nc, United States, duke center for applied genomics and precision medicine,durham,nc, United States, department of biology,duke university,durham,nc, United States, department of genetics,university of cambridge,cambridge,united kingdom,department of applied maths and theoretical physics,centre for mathematical sciences,wilberforce road,university of cambridge,cambridge, United Kingdom
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Authors
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