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UGGT1 enhances enterovirus 71 pathogenicity by promoting viral RNA synthesis and viral replication
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نویسنده
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huang p.-n. ,jheng j.-r. ,arnold j.j. ,wang j.-r. ,cameron c.e. ,shih s.-r.
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منبع
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plos pathogens - 2017 - دوره : 13 - شماره : 5
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چکیده
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Positive-strand rna virus infections can induce the stress-related unfolded protein response (upr) in host cells. this study found that enterovirus a71 (eva71) utilizes host udp-glucose glycoprotein glucosyltransferase 1 (uggt1),a key endoplasmic reticulum protein (er) involved in upr,to enhance viral replication and virulence. eva71 forms replication complexes (rcs) on cellular membranes that contain a mix of host and viral proteins to facilitate viral replication,but the components and processes involved in the assembly and function of rcs are not fully understood. using eva71 as a model,this study found that host uggt1 and viral 3d polymerase co-precipitate along with other factors on membranous replication complexes to enhance viral replication. increased uggt1 levels elevated viral growth rates,while viral pathogenicity was observed to be lower in heterozygous knockout mice (uggt1 +/- mice). these findings provide important insight on the role of upr and host uggt1 in regulating rna virus replication and pathogenicity. © 2017 huang et al.
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آدرس
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research center for emerging viral infections,college of medicine,chang gung university,taoyuan,taiwan,department of medical biotechnology and laboratory science,college of medicine,chang gung university,taoyuan, Taiwan, research center for emerging viral infections,college of medicine,chang gung university,taoyuan,taiwan,graduate institute of biomedical science,college of medicine,chang gung university,taoyuan, Taiwan, department of biochemistry and molecular biology,pennsylvania state university,university park,pa, United States, department of medical laboratory science and biotechnology,national cheng kung university,tainan, Taiwan, department of biochemistry and molecular biology,pennsylvania state university,university park,pa, United States, research center for emerging viral infections,college of medicine,chang gung university,taoyuan,taiwan,department of medical biotechnology and laboratory science,college of medicine,chang gung university,taoyuan,taiwan,graduate institute of biomedical science,college of medicine,chang gung university,taoyuan,taiwan,clinical virology laboratory,department of laboratory medicine,chang gung memorial hospital,taoyuan, Taiwan
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Authors
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