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A unique profilin-actin interface is important for malaria parasite motility
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نویسنده
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moreau c.a. ,bhargav s.p. ,kumar h. ,quadt k.a. ,piirainen h. ,strauss l. ,kehrer j. ,streichfuss m. ,spatz j.p. ,wade r.c. ,kursula i. ,frischknecht f.
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منبع
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plos pathogens - 2017 - دوره : 13 - شماره : 5
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چکیده
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Profilin is an actin monomer binding protein that provides atp-actin for incorporation into actin filaments. in contrast to higher eukaryotic cells with their large filamentous actin structures,apicomplexan parasites typically contain only short and highly dynamic microfilaments. in apicomplexans,profilin appears to be the main monomer-sequestering protein. compared to classical profilins,apicomplexan profilins contain an additional arm-like β-hairpin motif,which we show here to be critically involved in actin binding. through comparative analysis using two profilin mutants,we reveal this motif to be implicated in gliding motility of plasmodium berghei sporozoites,the rapidly migrating forms of a rodent malaria parasite transmitted by mosquitoes. force measurements on migrating sporozoites and molecular dynamics simulations indicate that the interaction between actin and profilin fine-tunes gliding motility. our data suggest that evolutionary pressure to achieve efficient high-speed gliding has resulted in a unique profilin-actin interface in these parasites. © 2017 moreau et al.
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آدرس
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integrative parasitology,center for infectious diseases,heidelberg university medical school,heidelberg, Germany, integrative parasitology,center for infectious diseases,heidelberg university medical school,heidelberg,germany,biocenter oulu and faculty of biochemistry and molecular medicine,university of oulu,oulu, Finland, integrative parasitology,center for infectious diseases,heidelberg university medical school,heidelberg,germany,molecular and cellular modeling group,heidelberg institute for theoretical studies (hits),heidelberg, Germany, integrative parasitology,center for infectious diseases,heidelberg university medical school,heidelberg,germany,institute for physical chemistry,biophysical chemistry,heidelberg university,heidelberg, Germany, biocenter oulu and faculty of biochemistry and molecular medicine,university of oulu,oulu, Finland, integrative parasitology,center for infectious diseases,heidelberg university medical school,heidelberg, Germany, integrative parasitology,center for infectious diseases,heidelberg university medical school,heidelberg, Germany, integrative parasitology,center for infectious diseases,heidelberg university medical school,heidelberg,germany,institute for physical chemistry,biophysical chemistry,heidelberg university,heidelberg, Germany, institute for physical chemistry,biophysical chemistry,heidelberg university,heidelberg,germany,department of cellular biophysics,max-planck institute for medical research,heidelberg, Germany, molecular and cellular modeling group,heidelberg institute for theoretical studies (hits),heidelberg,germany,center for molecular biology (zmbh),dkfz-zmbh alliance and interdisciplinary center for scientific computing (iwr),heidelberg university,heidelberg, Germany, biocenter oulu and faculty of biochemistry and molecular medicine,university of oulu,oulu,finland,department of biomedicine,university of bergen,bergen, Norway, integrative parasitology,center for infectious diseases,heidelberg university medical school,heidelberg, Germany
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Authors
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