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Rare HIV-1 transmitted/founder lineages identified by deep viral sequencing contribute to rapid shifts in dominant quasispecies during acute and early infection
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نویسنده
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kijak g.h. ,sanders-buell e. ,chenine a.-l. ,eller m.a. ,goonetilleke n. ,thomas r. ,leviyang s. ,harbolick e.a. ,bose m. ,pham p. ,oropeza c. ,poltavee k. ,o’sullivan a.m. ,billings e. ,merbah m. ,costanzo m.c. ,warren j.a. ,slike b. ,li h. ,peachman k.k. ,fischer w. ,gao f. ,cicala c. ,arthos j. ,eller l.a. ,o’connell r.j. ,sinei s. ,maganga l. ,kibuuka h. ,nitayaphan s. ,rao m. ,marovich m.a. ,krebs s.j. ,rolland m. ,korber b.t. ,shaw g.m. ,michael n.l. ,robb m.l. ,tovanabutra s. ,kim j.h.
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منبع
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plos pathogens - 2017 - دوره : 13 - شماره : 7
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چکیده
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In order to inform the rational design of hiv-1 preventive and cure interventions it is critical to understand the events occurring during acute hiv-1 infection (ahi). using viral deep sequencing on six participants from the early capture acute infection rv217 cohort,we have studied hiv-1 evolution in plasma collected twice weekly during the first weeks following the advent of viremia. the analysis of infections established by multiple transmitted/founder (t/f) viruses revealed novel viral profiles that included: a) the low-level persistence of minor t/f variants,b) the rapid replacement of the major t/f by a minor t/f,and c) an initial expansion of the minor t/f followed by a quick collapse of the same minor t/f to low frequency. in most participants,cytotoxic t-lymphocyte (ctl) escape was first detected at the end of peak viremia downslope,proceeded at higher rates than previously measured in hiv-1 infection,and usually occurred through the exploration of multiple mutational pathways within an epitope. the rapid emergence of ctl escape variants suggests a strong and early ctl response. minor t/f viral strains can contribute to rapid and varied profiles of hiv-1 quasispecies evolution during ahi. overall,our results demonstrate that early,deep,and frequent sampling is needed to investigate viral/host interaction during ahi,which could help identify prerequisites for prevention and cure of hiv-1 infection. © 2017 public library of science. all rights reserved.
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آدرس
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u.s. military hiv research program,walter reed army institute of research,silver spring,md,united states,henry m. jackson foundation for the advancement of military medicine,bethesda,md,united states,gsk vaccines,rockville,md, United States, u.s. military hiv research program,walter reed army institute of research,silver spring,md,united states,henry m. jackson foundation for the advancement of military medicine,bethesda,md, United States, u.s. military hiv research program,walter reed army institute of research,silver spring,md,united states,henry m. jackson foundation for the advancement of military medicine,bethesda,md, United States, u.s. military hiv research program,walter reed army institute of research,silver spring,md,united states,henry m. jackson foundation for the advancement of military medicine,bethesda,md, United States, school of medicine,the university of north carolina at chapel hill,chapel hill,nc, United States, u.s. military hiv research program,walter reed army institute of research,silver spring,md,united states,henry m. jackson foundation for the advancement of military medicine,bethesda,md, United States, department of mathematics and statistics,georgetown university,washington,dc, United States, u.s. military hiv research program,walter reed army institute of research,silver spring,md,united states,henry m. jackson foundation for the advancement of military medicine,bethesda,md, United States, u.s. military hiv research program,walter reed army institute of research,silver spring,md,united states,henry m. jackson foundation for the advancement of military medicine,bethesda,md, United States, u.s. military hiv research program,walter reed army institute of research,silver spring,md,united states,henry m. jackson foundation for the advancement of military medicine,bethesda,md, United States, u.s. military hiv research program,walter reed army institute of research,silver spring,md,united states,henry m. jackson foundation for the advancement of military medicine,bethesda,md, United States, u.s. military hiv research program,walter reed army institute of research,silver spring,md,united states,henry m. jackson foundation for the advancement of military medicine,bethesda,md, United States, u.s. military hiv research program,walter reed army institute of research,silver spring,md,united states,henry m. jackson foundation for the advancement of military medicine,bethesda,md, United States, u.s. military hiv research program,walter reed army institute of research,silver spring,md,united states,henry m. jackson foundation for the advancement of military medicine,bethesda,md, United States, u.s. military hiv research program,walter reed army institute of research,silver spring,md,united states,henry m. jackson foundation for the advancement of military medicine,bethesda,md, United States, u.s. military hiv research program,walter reed army institute of research,silver spring,md,united states,henry m. jackson foundation for the advancement of military medicine,bethesda,md, United States, school of medicine,the university of north carolina at chapel hill,chapel hill,nc, United States, u.s. military hiv research program,walter reed army institute of research,silver spring,md,united states,henry m. jackson foundation for the advancement of military medicine,bethesda,md, United States, perelman school of medicine,university of pennsylvania,philadelphia,pa, United States, u.s. military hiv research program,walter reed army institute of research,silver spring,md,united states,henry m. jackson foundation for the advancement of military medicine,bethesda,md, United States, theoretical biology,los alamos national laboratory,los alamos,nm, United States, duke human vaccine institute,duke university medical center,durham,nc, United States, laboratory of immunoregulation national institute of allergy and infectious diseases,national institutes of health,bethesda,md, United States, laboratory of immunoregulation national institute of allergy and infectious diseases,national institutes of health,bethesda,md, United States, u.s. military hiv research program,walter reed army institute of research,silver spring,md,united states,henry m. jackson foundation for the advancement of military medicine,bethesda,md, United States, armed forces research institute of medical sciences,bangkok, Thailand, walter reed project,kericho, Kenya, mbeya medical research programme,mbeya, Tanzania, makerere university-walter reed project,kampala, Uganda, armed forces research institute of medical sciences,bangkok, Thailand, u.s. military hiv research program,walter reed army institute of research,silver spring,md, United States, vaccine research program,national institute of allergy and infectious diseases,national institutes of health,rockville,md, United States, u.s. military hiv research program,walter reed army institute of research,silver spring,md,united states,henry m. jackson foundation for the advancement of military medicine,bethesda,md, United States, u.s. military hiv research program,walter reed army institute of research,silver spring,md,united states,henry m. jackson foundation for the advancement of military medicine,bethesda,md, United States, theoretical biology,los alamos national laboratory,los alamos,nm, United States, perelman school of medicine,university of pennsylvania,philadelphia,pa, United States, u.s. military hiv research program,walter reed army institute of research,silver spring,md, United States, u.s. military hiv research program,walter reed army institute of research,silver spring,md,united states,henry m. jackson foundation for the advancement of military medicine,bethesda,md, United States, u.s. military hiv research program,walter reed army institute of research,silver spring,md,united states,henry m. jackson foundation for the advancement of military medicine,bethesda,md, United States, u.s. military hiv research program,walter reed army institute of research,silver spring,md,united states,international vaccine institute,seoul, South Korea
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Authors
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