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Comparative genomics of geographically distant Fusarium fujikuroi isolates revealed two distinct pathotypes correlating with secondary metabolite profiles
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نویسنده
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niehaus e.-m. ,kim h.-k. ,münsterkötter m. ,janevska s. ,arndt b. ,kalinina s.a. ,houterman p.m. ,ahn i.-p. ,alberti i. ,tonti s. ,kim d.-w. ,sieber c.m.k. ,humpf h.-u. ,yun s.-h. ,güldener u. ,tudzynski b.
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منبع
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plos pathogens - 2017 - دوره : 13 - شماره : 10
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چکیده
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Fusarium fujikuroi causes bakanae (“foolish seedling”) disease of rice which is characterized by hyper-elongation of seedlings resulting from production of gibberellic acids (gas) by the fungus. this plant pathogen is also known for production of harmful mycotoxins,such as fusarins,fusaric acid,apicidin f and beauvericin. recently,we generated the first de novo genome sequence of f. fujikuroi strain imi 58289 combined with extensive transcriptional,epigenetic,proteomic and chemical product analyses. ga production was shown to provide a selective advantage during infection of the preferred host plant rice. here,we provide genome sequences of eight additional f. fujikuroi isolates from distant geographic regions. the isolates differ in the size of chromosomes,most likely due to variability of subtelomeric regions,the type of asexual spores (microconidia and/or macroconidia),and the number and expression of secondary metabolite gene clusters. whilst most of the isolates caused the typical bakanae symptoms,one isolate,b14,caused stunting and early withering of infected seedlings. in contrast to the other isolates,b14 produced no gas but high amounts of fumonisins during infection on rice. furthermore,it differed from the other isolates by the presence of three additional polyketide synthase (pks) genes (pks40,pks43,pks51) and the absence of the f. fujikuroi-specific apicidin f (nrps31) gene cluster. analysis of additional field isolates confirmed the strong correlation between the pathotype (bakanae or stunting/withering),and the ability to produce either gas or fumonisins. deletion of the fumonisin and fusaric acid-specific pks genes in b14 reduced the stunting/withering symptoms,whereas deletion of the pks51 gene resulted in elevated symptom development. phylogenetic analyses revealed two subclades of f. fujikuroi strains according to their pathotype and secondary metabolite profiles. © 2017 niehaus et al.
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آدرس
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institute of biology and biotechnology of plants,molecular biology and biotechnology of fungi,westfälische wilhelms-universität münster,münster,germany,institute of food chemistry,westfälische wilhelms-universität münster,corrensstraße,münster, Germany, department of medical biotechnology,soonchunhyang university,asan, South Korea, institute of bioinformatics and systems biology,helmholtz zentrum münchen,german research center for environmental health (gmbh),neuherberg, Germany, institute of biology and biotechnology of plants,molecular biology and biotechnology of fungi,westfälische wilhelms-universität münster,münster, Germany, institute of food chemistry,westfälische wilhelms-universität münster,corrensstraße 45,münster, Germany, institute of food chemistry,westfälische wilhelms-universität münster,corrensstraße 45,münster, Germany, university of amsterdam,swammerdam institute for life sciences,plant pathology,amsterdam, Netherlands, department of agricultural biotechnology,national institute of agricultural sciences,rural development administration,wanju, South Korea, crea-cin sede di rovigo,viale giovanni amendola 82,rovigo,45100, Italy, crea-scs sede di bologna,via di corticella 133,bologna,40128, Italy, department of medical biotechnology,soonchunhyang university,asan, South Korea, department of energy joint genome institute,university of california,walnut creek,berkeley,ca, United States, institute of food chemistry,westfälische wilhelms-universität münster,corrensstraße 45,münster, Germany, department of medical biotechnology,soonchunhyang university,asan, South Korea, institute of bioinformatics and systems biology,helmholtz zentrum münchen,german research center for environmental health (gmbh),neuherberg,germany,chair of genome-oriented bioinformatics,tum school of life sciences weihenstephan,technical university of munich,freising, Germany, institute of biology and biotechnology of plants,molecular biology and biotechnology of fungi,westfälische wilhelms-universität münster,münster, Germany
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Authors
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