Enterovirus 71 protease 2Aproand 3Cprodifferentially inhibit the cellular endoplasmic reticulum-associated degradation (ERAD) pathway via distinct mechanisms,and enterovirus 71 hijacks ERAD component p97 to promote its replication

Endoplasmic reticulum-associated degradation (erad) is an important function for cellular homeostasis. the mechanism of how picornavirus infection interferes with erad remains unclear. in this study,we demonstrated that enterovirus 71 (ev71) infection significantly inhibits cellular erad by targeting multiple key erad molecules with its proteases 2aproand 3cprousing different mechanisms. ubc6e was identified as the key e2 ubiquitin-conjugating enzyme in ev71 disturbed erad. ev71 3cprocleaves ubc6e at q219g,q260s,and q273g. ev71 2apromainly inhibits the de novo synthesis of key erad molecules herp and vimp at the protein translational level. herp differentially participates in the degradation of different glycosylated erad substrates α-1 antitrypsin null hong kong (nhk) and the c-terminus of sonic hedgehog (shh-c) via unknown mechanisms. p97 was identified as a host factor in ev71 replication; it redistributed and co-exists with the viral protein and other known replication-related molecules in ev71-induced replication organelles. electron microscopy and multiple-color confocal assays also showed that ev71-induced membranous vesicles were closely associated with the endoplasmic reticulum (er),and the er membrane molecule rtn3 was redistributed to the viral replication complex during ev71 infection. therefore,we propose that ev71 rearranges er membranes and hijacks p97 from cellular erad to benefit its replication. these findings add to our understanding of how viruses disturb erad and provide potential anti-viral targets for ev71 infection. © 2017 wang et al.