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Novel Marmoset (Callithrix jacchus) Model of Human Herpesvirus 6A and 6B Infections: Immunologic,Virologic and Radiologic Characterization
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نویسنده
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leibovitch e. ,wohler j.e. ,cummings macri s.m. ,motanic k. ,harberts e. ,gaitán m.i. ,maggi p. ,ellis m. ,westmoreland s. ,silva a. ,reich d.s. ,jacobson s.
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منبع
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plos pathogens - 2013 - دوره : 9 - شماره : 1
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چکیده
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Human herpesvirus 6 (hhv-6) is a ubiquitous virus with an estimated seroprevalence of 95% in the adult population. hhv-6 is associated with several neurologic disorders,including multiple sclerosis,an inflammatory demyelinating disease affecting the cns. animal models of hhv-6 infection would help clarify its role in human disease but have been slow to develop because rodents lack cd46,the receptor for cellular entry. therefore,we investigated the effects of hhv-6 infections in a non-human primate,the common marmoset callithrix jacchus. we inoculated a total of 12 marmosets with hhv-6a and hhv-6b intravenously and hhv-6a intranasally. animals were monitored for 25 weeks post-inoculation clinically,immunologically and by mri. marmosets inoculated with hhv-6a intravenously exhibited neurologic symptoms and generated virus-specific antibody responses,while those inoculated intravenously with hhv-6b were asymptomatic and generated comparatively lower antibody responses. viral dna was detected at a low frequency in paraffin-embedded cns tissue of a subset of marmosets inoculated with hhv-6a and hhv-6b intravenously. when different routes of hhv-6a inoculation were compared,intravenous inoculation resulted in virus-specific antibody responses and infrequent detection of viral dna in the periphery,while intranasal inoculation resulted in negligible virus-specific antibody responses and frequent detection of viral dna in the periphery. moreover,marmosets inoculated with hhv-6a intravenously exhibited neurologic symptoms,while marmosets inoculated with hhv-6a intranasally were asymptomatic. we demonstrate that a marmoset model of hhv-6 infection can serve to further define the contribution of this ubiquitous virus to human neurologic disorders.
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آدرس
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viral immunology section,neuroimmunology branch,ninds/nih,bethesda,md,united states,institute for biomedical sciences,george washington university,washington,dc, United States, viral immunology section,neuroimmunology branch,ninds/nih,bethesda,md, United States, new england primate research center,harvard medical school,southborough,ma, United States, viral immunology section,neuroimmunology branch,ninds/nih,bethesda,md, United States, viral immunology section,neuroimmunology branch,ninds/nih,bethesda,md, United States, translational neuroradiology unit,neuroimmunology branch,ninds/nih,bethesda,md, United States, translational neuroradiology unit,neuroimmunology branch,ninds/nih,bethesda,md, United States, new england primate research center,harvard medical school,southborough,ma, United States, new england primate research center,harvard medical school,southborough,ma, United States, cerebral microcirculation unit,laboratory of functional and molecular imaging,ninds/nih,bethesda,md, United States, translational neuroradiology unit,neuroimmunology branch,ninds/nih,bethesda,md, United States, viral immunology section,neuroimmunology branch,ninds/nih,bethesda,md, United States
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Authors
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