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Evidence for Novel Hepaciviruses in Rodents
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نویسنده
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drexler j.f. ,corman v.m. ,müller m.a. ,lukashev a.n. ,gmyl a. ,coutard b. ,adam a. ,ritz d. ,leijten l.m. ,van riel d. ,kallies r. ,klose s.m. ,gloza-rausch f. ,binger t. ,annan a. ,adu-sarkodie y. ,oppong s. ,bourgarel m. ,rupp d. ,hoffmann b. ,schlegel m. ,kümmerer b.m. ,krüger d.h. ,schmidt-chanasit j. ,setién a.a. ,cottontail v.m. ,hemachudha t. ,wacharapluesadee s. ,osterrieder k. ,bartenschlager r. ,matthee s. ,beer m. ,kuiken t. ,reusken c. ,leroy e.m. ,ulrich r.g. ,drosten c.
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منبع
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plos pathogens - 2013 - دوره : 9 - شماره : 6
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چکیده
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Hepatitis c virus (hcv) is among the most relevant causes of liver cirrhosis and hepatocellular carcinoma. research is complicated by a lack of accessible small animal models. the systematic investigation of viruses of small mammals could guide efforts to establish such models,while providing insight into viral evolutionary biology. we have assembled the so-far largest collection of small-mammal samples from around the world,qualified to be screened for bloodborne viruses,including sera and organs from 4,770 rodents (41 species); and sera from 2,939 bats (51 species). three highly divergent rodent hepacivirus clades were detected in 27 (1.8%) of 1,465 european bank voles (myodes glareolus) and 10 (1.9%) of 518 south african four-striped mice (rhabdomys pumilio). bats showed anti-hcv immunoblot reactivities but no virus detection,although the genetic relatedness suggested by the serologic results should have enabled rna detection using the broadly reactive pcr assays developed for this study. 210 horses and 858 cats and dogs were tested,yielding further horse-associated hepaciviruses but none in dogs or cats. the rodent viruses were equidistant to hcv,exceeding by far the diversity of hcv and the canine/equine hepaciviruses taken together. five full genomes were sequenced,representing all viral lineages. salient genome features and distance criteria supported classification of all viruses as hepaciviruses. quantitative rt-pcr,rna in-situ hybridisation,and histopathology suggested hepatic tropism with liver inflammation resembling hepatitis c. recombinant serology for two distinct hepacivirus lineages in 97 bank voles identified seroprevalence rates of 8.3 and 12.4%,respectively. antibodies in bank vole sera neither cross-reacted with hcv,nor the heterologous bank vole hepacivirus. co-occurrence of rna and antibodies was found in 3 of 57 pcr-positive bank vole sera (5.3%). our data enable new hypotheses regarding hcv evolution and encourage efforts to develop rodent surrogate models for hcv. © 2013 drexler et al.
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آدرس
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institute of virology,university of bonn medical centre,bonn, Germany, institute of virology,university of bonn medical centre,bonn, Germany, institute of virology,university of bonn medical centre,bonn, Germany, chumakov institute of poliomyelitis and viral encephalitides,moscow, Russian Federation, chumakov institute of poliomyelitis and viral encephalitides,moscow,russian federation,lomonosov moscow state university,moscow, Russian Federation, architectures et fonctions des macromolécules biologiques,umr 7257 cnrs and aix-marseille university,marseille, France, institute of pathology,university of cologne medical centre,cologne, Germany, institute of virology,university of bonn medical centre,bonn, Germany, erasmus mc,department of viroscience,rotterdam, Netherlands, erasmus mc,department of viroscience,rotterdam, Netherlands, institute of virology,university of bonn medical centre,bonn, Germany, institute of experimental ecology,university of ulm,ulm, Germany, institute of virology,university of bonn medical centre,bonn,germany,noctalis,centre for bat protection and information,bad segeberg, Germany, institute of virology,university of bonn medical centre,bonn, Germany, kumasi centre for collaborative research in tropical medicine (kccr),kumasi, Ghana, kwame nkrumah university of science and technology,kumasi, Ghana, kwame nkrumah university of science and technology,kumasi, Ghana, centre de cooperation internationale de recherche en agronomie pour le développement,upr agirs,montpellier, France, department of infectious diseases,molecular virology,medical facility,heidelberg university,heidelberg, Germany, friedrich-loeffler-institut,institute for virus diagnostics,greifswald-insel riems, Germany, friedrich-loeffler-institut,institute for novel and emerging infectious diseases,greifswald-insel riems, Germany, institute of virology,university of bonn medical centre,bonn, Germany, institute of medical virology (helmut ruska haus),charité medical school,berlin, Germany, bernhard nocht institute for tropical medicine,department of virology,hamburg, Germany, unidad de investigación médica en inmunología,hospital de pediatría,méxico df, Mexico, institute of experimental ecology,university of ulm,ulm, Germany, chulalongkorn university,faculty of medicine,neuroscience center for research and development,bangkok, Thailand, chulalongkorn university,faculty of medicine,neuroscience center for research and development,bangkok, Thailand, institute of virology,free university of berlin,department of veterinary medicine,berlin, Germany, department of infectious diseases,molecular virology,medical facility,heidelberg university,heidelberg, Germany, department of conservation ecology and entomology,stellenbosch university,stellenbosch, South Africa, friedrich-loeffler-institut,institute for virus diagnostics,greifswald-insel riems, Germany, erasmus mc,department of viroscience,rotterdam, Netherlands, netherlands center for infectious disease control,bilthoven, Netherlands, centre international de recherches médicales de franceville,franceville,gabon,institut de recherche pour le développement,umr 224 (mivegec),ird/cnrs/um1,montpellier, France, friedrich-loeffler-institut,institute for novel and emerging infectious diseases,greifswald-insel riems, Germany, institute of virology,university of bonn medical centre,bonn, Germany
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