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A Type IV Pilus Mediates DNA Binding during Natural Transformation in Streptococcus pneumoniae
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نویسنده
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laurenceau r. ,péhau-arnaudet g. ,baconnais s. ,gault j. ,malosse c. ,dujeancourt a. ,campo n. ,chamot-rooke j. ,le cam e. ,claverys j.-p. ,fronzes r.
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منبع
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plos pathogens - 2013 - دوره : 9 - شماره : 6
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چکیده
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Natural genetic transformation is widely distributed in bacteria and generally occurs during a genetically programmed differentiated state called competence. this process promotes genome plasticity and adaptability in gram-negative and gram-positive bacteria. transformation requires the binding and internalization of exogenous dna,the mechanisms of which are unclear. here,we report the discovery of a transformation pilus at the surface of competent streptococcus pneumoniae cells. this type iv-like pilus,which is primarily composed of the comgc pilin,is required for transformation. we provide evidence that it directly binds dna and propose that the transformation pilus is the primary dna receptor on the bacterial cell during transformation in s. pneumoniae. being a central component of the transformation apparatus,the transformation pilus enables s. pneumoniae,a major gram-positive human pathogen,to acquire resistance to antibiotics and to escape vaccines through the binding and incorporation of new genetic material. © 2013 laurenceau et al.
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آدرس
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institut pasteur,groupe biologie structurale de la sécrétion bactérienne,paris,france,cnrs,umr3528,paris, France, cnrs,umr3528,paris, France, maintenance des génomes et microscopies moléculaire,umr 8126 cnrs-université paris sud,institut gustave roussy,villejuif, France, cnrs,umr3528,paris,france,institut pasteur,unité de spectrométrie de masse structurale et protéomique,paris, France, cnrs,umr3528,paris,france,institut pasteur,unité de spectrométrie de masse structurale et protéomique,paris, France, institut pasteur,groupe biologie structurale de la sécrétion bactérienne,paris,france,cnrs,umr3528,paris, France, cnrs,umr5100,toulouse,france,université de toulouse,ups,laboratoire de microbiologie et génétique moléculaires,toulouse, France, cnrs,umr3528,paris,france,institut pasteur,unité de spectrométrie de masse structurale et protéomique,paris, France, maintenance des génomes et microscopies moléculaire,umr 8126 cnrs-université paris sud,institut gustave roussy,villejuif, France, cnrs,umr5100,toulouse,france,université de toulouse,ups,laboratoire de microbiologie et génétique moléculaires,toulouse, France, institut pasteur,groupe biologie structurale de la sécrétion bactérienne,paris,france,cnrs,umr3528,paris, France
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Authors
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