|
|
|
|
Novel Polyomaviruses of Nonhuman Primates: Genetic and Serological Predictors for the Existence of Multiple Unknown Polyomaviruses within the Human Population
|
|
|
|
|
|
|
|
نویسنده
|
scuda n. ,madinda n.f. ,akoua-koffi c. ,adjogoua e.v. ,wevers d. ,hofmann j. ,cameron k.n. ,leendertz s.a.j. ,couacy-hymann e. ,robbins m. ,boesch c. ,jarvis m.a. ,moens u. ,mugisha l. ,calvignac-spencer s. ,leendertz f.h. ,ehlers b.
|
|
منبع
|
plos pathogens - 2013 - دوره : 9 - شماره : 6
|
|
چکیده
|
Polyomaviruses are a family of small non-enveloped dna viruses that encode oncogenes and have been associated,to greater or lesser extent,with human disease and cancer. currently,twelve polyomaviruses are known to circulate within the human population. to further examine the diversity of human polyomaviruses,we have utilized a combinatorial approach comprised of initial degenerate primer-based pcr identification and phylogenetic analysis of nonhuman primate (nhp) polyomavirus species,followed by polyomavirus-specific serological analysis of human sera. using this approach we identified twenty novel nhp polyomaviruses: nine in great apes (six in chimpanzees,two in gorillas and one in orangutan),five in old world monkeys and six in new world monkeys. phylogenetic analysis indicated that only four of the nine chimpanzee polyomaviruses (six novel and three previously identified) had known close human counterparts. to determine whether the remaining chimpanzee polyomaviruses had potential human counterparts,the major viral capsid proteins (vp1) of four chimpanzee polyomaviruses were expressed in e. coli for use as antigens in enzyme-linked immunoassay (elisa). human serum/plasma samples from both côte d'ivoire and germany showed frequent seropositivity for the four viruses. antibody pre-adsorption-based elisa excluded the possibility that reactivities resulted from binding to known human polyomaviruses. together,these results support the existence of additional polyomaviruses circulating within the human population that are genetically and serologically related to existing chimpanzee polyomaviruses. © 2013 scuda et al.
|
|
|
|
|
آدرس
|
department of infectious diseases,robert koch institute,berlin, Germany, project 23 epidemiology of highly pathogenic microorganisms,robert koch institute,berlin,germany,department of primatology,max planck institute,for evolutionary anthropology,leipzig, Germany, university teaching hospital bouaké,bouaké, Cote d'Ivoire, institut pasteur côte d'ivoire,abidjan, Cote d'Ivoire, department of infectious diseases,robert koch institute,berlin, Germany, institute of virology,charité - universitätsmedizin berlin,berlin, Germany, mountain gorilla veterinary project,inc.,maryland,baltimore,md,united states,wildlife conservation society,new york,ny, United States, project 23 epidemiology of highly pathogenic microorganisms,robert koch institute,berlin, Germany, lanada/laboratoire central de la pathologie animale,bingerville, Cote d'Ivoire, department of primatology,max planck institute,for evolutionary anthropology,leipzig, Germany, department of primatology,max planck institute,for evolutionary anthropology,leipzig, Germany, school of biomedical and biological sciences,university of plymouth,plymouth, United Kingdom, university of tromsø,faculty of health sciences,department of medical biology,tromsø, Norway, ecohealth research group,conservation and ecosystem health alliance (ceha),kampala, Uganda, project 23 epidemiology of highly pathogenic microorganisms,robert koch institute,berlin, Germany, project 23 epidemiology of highly pathogenic microorganisms,robert koch institute,berlin, Germany, department of infectious diseases,robert koch institute,berlin, Germany
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Authors
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|