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Real-Time Whole-Body Visualization of Chikungunya Virus Infection and Host Interferon Response in Zebrafish
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نویسنده
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palha n. ,guivel-benhassine f. ,briolat v. ,lutfalla g. ,sourisseau m. ,ellett f. ,wang c.-h. ,lieschke g.j. ,herbomel p. ,schwartz o. ,levraud j.-p.
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منبع
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plos pathogens - 2013 - دوره : 9 - شماره : 9
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چکیده
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Chikungunya virus (chikv),a re-emerging arbovirus that may cause severe disease,constitutes an important public health problem. herein we describe a novel chikv infection model in zebrafish,where viral spread was live-imaged in the whole body up to cellular resolution. infected cells emerged in various organs in one principal wave with a median appearance time of ∼14 hours post infection. timing of infected cell death was organ dependent,leading to a shift of chikv localization towards the brain. as in mammals,chikv infection triggered a strong type-i interferon (ifn) response,critical for survival. ifn was mainly expressed by neutrophils and hepatocytes. cell type specific ablation experiments further demonstrated that neutrophils play a crucial,unexpected role in chikv containment. altogether,our results show that the zebrafish represents a novel valuable model to dynamically visualize replication,pathogenesis and host responses to a human virus. © 2013 palha et al.
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آدرس
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institut pasteur,macrophages et développement de l'immunité,department of developmental and stem cells biology,paris,france,cnrs ura2578,paris,france,université pierre et marie curie,paris, France, institut pasteur,virus et immunité,department of virology,paris,france,cnrs ura3015,paris, France, institut pasteur,macrophages et développement de l'immunité,department of developmental and stem cells biology,paris,france,cnrs ura2578,paris, France, cnrs umr5235,dynamiques des interactions membranaires et pathologiques,montpellier,france,université montpellier ii,montpellier, France, institut pasteur,virus et immunité,department of virology,paris,france,cnrs ura3015,paris,france,mount sinai school of medicine,new york,ny, United States, australian regenerative medicine institute,monash university,clayton,vic,australia,mrc centre for developmental and biomedical genetics,sheffield, United Kingdom, australian regenerative medicine institute,monash university,clayton,vic, Australia, australian regenerative medicine institute,monash university,clayton,vic, Australia, institut pasteur,macrophages et développement de l'immunité,department of developmental and stem cells biology,paris,france,cnrs ura2578,paris, France, institut pasteur,virus et immunité,department of virology,paris,france,cnrs ura3015,paris, France, institut pasteur,macrophages et développement de l'immunité,department of developmental and stem cells biology,paris,france,cnrs ura2578,paris, France
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Authors
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