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Structural and Biochemical Characterization Reveals LysGH15 as an Unprecedented EF-Hand-Like Calcium-Binding Phage Lysin
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نویسنده
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gu j. ,feng y. ,feng x. ,sun c. ,lei l. ,ding w. ,niu f. ,jiao l. ,yang m. ,li y. ,liu x. ,song j. ,cui z. ,han d. ,du c. ,yang y. ,ouyang s. ,liu z.-j. ,han w.
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منبع
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plos pathogens - 2014 - دوره : 10 - شماره : 5
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چکیده
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The lysin lysgh15,which is derived from the staphylococcal phage gh15,demonstrates a wide lytic spectrum and strong lytic activity against methicillin-resistant staphylococcus aureus (mrsa). here,we find that the lytic activity of the full-length lysgh15 and its chap domain is dependent on calcium ions. to elucidate the molecular mechanism,the structures of three individual domains of lysgh15 were determined. unexpectedly,the crystal structure of the lysgh15 chap domain reveals an ef-hand-like calcium-binding site near the cys-his-glu-asn quartet active site groove. to date,the calcium-binding site in the lysgh15 chap domain is unique among homologous proteins,and it represents the first reported calcium-binding site in the chap family. more importantly,the calcium ion plays an important role as a switch that modulates the chap domain between the active and inactive states. structure-guided mutagenesis of the amidase-2 domain reveals that both the zinc ion and e282 are required in catalysis and enable us to propose a catalytic mechanism. nuclear magnetic resonance (nmr) spectroscopy and titration-guided mutagenesis identify residues (e.g.,n404,y406,g407,and t408) in the sh3b domain that are involved in the interactions with the substrate. to the best of our knowledge,our results constitute the first structural information on the biochemical features of a staphylococcal phage lysin and represent a pivotal step forward in understanding this type of lysin. © 2014 gu et al.
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آدرس
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key laboratory of zoonosis,ministry of education,college of veterinary medicine,jilin university,changchun, China, shandong provincial key laboratory of energy genetics,qingdao institute of bioenergy and bioprocess technology,chinese academy of sciences,qingdao, China, key laboratory of zoonosis,ministry of education,college of veterinary medicine,jilin university,changchun, China, key laboratory of zoonosis,ministry of education,college of veterinary medicine,jilin university,changchun, China, key laboratory of zoonosis,ministry of education,college of veterinary medicine,jilin university,changchun, China, center for biological imaging,institute of biophysics,chinese academy of sciences,beijing, China, national laboratory of biomacromolecules,institute of biophysics,chinese academy of sciences,beijing, China, national laboratory of biomacromolecules,institute of biophysics,chinese academy of sciences,beijing, China, key laboratory of zoonosis,ministry of education,college of veterinary medicine,jilin university,changchun, China, key laboratory of zoonosis,ministry of education,college of veterinary medicine,jilin university,changchun, China, key laboratory of zoonosis,ministry of education,college of veterinary medicine,jilin university,changchun, China, key laboratory of zoonosis,ministry of education,college of veterinary medicine,jilin university,changchun, China, key laboratory of zoonosis,ministry of education,college of veterinary medicine,jilin university,changchun, China, key laboratory of zoonosis,ministry of education,college of veterinary medicine,jilin university,changchun, China, key laboratory of zoonosis,ministry of education,college of veterinary medicine,jilin university,changchun, China, key laboratory of zoonosis,ministry of education,college of veterinary medicine,jilin university,changchun, China, national laboratory of biomacromolecules,institute of biophysics,chinese academy of sciences,beijing, China, national laboratory of biomacromolecules,institute of biophysics,chinese academy of sciences,beijing, China, key laboratory of zoonosis,ministry of education,college of veterinary medicine,jilin university,changchun, China
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Authors
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