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A Role for LHC1 in Higher Order Structure and Complement Binding of the Cryptococcus neoformans Capsule
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نویسنده
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park y.-d. ,shin s. ,panepinto j. ,ramos j. ,qiu j. ,frases s. ,albuquerque p. ,cordero r.j.b. ,zhang n. ,himmelreich u. ,beenhouwer d. ,bennett j.e. ,casadevall a. ,williamson p.r.
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منبع
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plos pathogens - 2014 - دوره : 10 - شماره : 5
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چکیده
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Polysaccharide capsules are important virulence factors for many microbial pathogens including the opportunistic fungus cryptococcus neoformans. in the present study,we demonstrate an unusual role for a secreted lactonohydrolase of c. neoformans,lhc1 in capsular higher order structure. analysis of extracted capsular polysaccharide from wild-type and lhc1δ strains by dynamic and static light scattering suggested a role for the lhc1 locus in altering the capsular polysaccharide,both reducing dimensions and altering its branching,density and solvation. these changes in the capsular structure resulted in lhc1-dependent alterations of antibody binding patterns,reductions in human and mouse complement binding and phagocytosis by the macrophage-like cell line j774,as well as increased virulence in mice. these findings identify a unique molecular mechanism for tertiary structural changes in a microbial capsule,facilitating immune evasion and virulence of a fungal pathogen.
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آدرس
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laboratory of clinical infectious diseases,national institute of allergy and infectious diseases,national institutes of health,bethesda,md, United States, section of infectious diseases,department of medicine,university of illinois at chicago college of medicine,chicago,il, United States, department of microbiology and immunology,university at buffalo,state university of new york,buffalo,ny, United States, section of infectious diseases,department of medicine,university of illinois at chicago college of medicine,chicago,il, United States, laboratory of clinical infectious diseases,national institute of allergy and infectious diseases,national institutes of health,bethesda,md, United States, department of microbiology and immunology,division of infectious diseases of the department of medicine,albert einstein college of medicine,bronx,ny,united states,laboratorio de ultraestrutura cellular hertha meyer,instituto de biofisica carlos chagas filho,universidade federal do rio de janeiro,rio de janeiro, Brazil, department of microbiology and immunology,division of infectious diseases of the department of medicine,albert einstein college of medicine,bronx,ny, United States, department of microbiology and immunology,division of infectious diseases of the department of medicine,albert einstein college of medicine,bronx,ny, United States, laboratory of clinical infectious diseases,national institute of allergy and infectious diseases,national institutes of health,bethesda,md, United States, biomedical nmr unit,department of medical diagnostic sciences,division of radiology,katholieke universiteit leuven,leuven, Belgium, division of infectious diseases,veterans affairs greater los angeles healthcare system,los angeles,ca,united states,department of medicine,david geffen school of medicine,university of california,los angeles,ca, United States, laboratory of clinical infectious diseases,national institute of allergy and infectious diseases,national institutes of health,bethesda,md, United States, department of microbiology and immunology,division of infectious diseases of the department of medicine,albert einstein college of medicine,bronx,ny, United States, laboratory of clinical infectious diseases,national institute of allergy and infectious diseases,national institutes of health,bethesda,md,united states,section of infectious diseases,department of medicine,university of illinois at chicago college of medicine,chicago,il, United States
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Authors
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