|
|
|
|
An Invertebrate Warburg Effect: A Shrimp Virus Achieves Successful Replication by Altering the Host Metabolome via the PI3K-Akt-mTOR Pathway
|
|
|
|
|
|
|
|
نویسنده
|
su m.-a. ,huang y.-t. ,chen i.-t. ,lee d.-y. ,hsieh y.-c. ,li c.-y. ,ng t.h. ,liang s.-y. ,lin s.-y. ,huang s.-w. ,chiang y.-a. ,yu h.-t. ,khoo k.-h. ,chang g.-d. ,lo c.-f. ,wang h.-c.
|
|
منبع
|
plos pathogens - 2014 - دوره : 10 - شماره : 6
|
|
چکیده
|
In this study,we used a systems biology approach to investigate changes in the proteome and metabolome of shrimp hemocytes infected by the invertebrate virus wssv (white spot syndrome virus) at the viral genome replication stage (12 hpi) and the late stage (24 hpi). at 12 hpi,but not at 24 hpi,there was significant up-regulation of the markers of several metabolic pathways associated with the vertebrate warburg effect (or aerobic glycolysis),including glycolysis,the pentose phosphate pathway,nucleotide biosynthesis,glutaminolysis and amino acid biosynthesis. we show that the pi3k-akt-mtor pathway was of central importance in triggering this wssv-induced warburg effect. although dsrna silencing of the mtorc1 activator rheb had only a relatively minor impact on wssv replication,in vivo chemical inhibition of akt,mtorc1 and mtorc2 suppressed the wssv-induced warburg effect and reduced both wssv gene expression and viral genome replication. when the warburg effect was suppressed by pretreatment with the mtor inhibitor torin 1,even the subsequent up-regulation of the tca cycle was insufficient to satisfy the virus's requirements for energy and macromolecular precursors. the wssv-induced warburg effect therefore appears to be essential for successful viral replication. © 2014 wang et al.
|
|
|
|
|
آدرس
|
institute of biotechnology,college of bioscience and biotechnology,national cheng kung university,tainan, Taiwan, institute of biotechnology,college of bioscience and biotechnology,national cheng kung university,tainan, Taiwan, institute of zoology,college of life science,national taiwan university,taipei, Taiwan, institute of biochemical sciences,college of life science,national taiwan university,taipei,taiwan,center for systems biology,national taiwan university,taipei, Taiwan, institute of biotechnology,college of bioscience and biotechnology,national cheng kung university,tainan, Taiwan, institute of biotechnology,college of bioscience and biotechnology,national cheng kung university,tainan, Taiwan, institute of biotechnology,college of bioscience and biotechnology,national cheng kung university,tainan, Taiwan, core facilities for protein structural analysis,institute of biological chemistry,academia sinica,taipei, Taiwan, academia sinica common mass spectrometry facilities at institute of biological chemistry,taipei, Taiwan, department of life science,college of life science,national taiwan university,taipei, Taiwan, institute of biotechnology,college of bioscience and biotechnology,national cheng kung university,tainan, Taiwan, department of life science,college of life science,national taiwan university,taipei, Taiwan, institute of biochemical sciences,college of life science,national taiwan university,taipei,taiwan,core facilities for protein structural analysis,institute of biological chemistry,academia sinica,taipei, Taiwan, institute of biochemical sciences,college of life science,national taiwan university,taipei, Taiwan, institute of zoology,college of life science,national taiwan university,taipei,taiwan,institute of bioinformatics and biosignal transduction,national cheng kung university,tainan, Taiwan, institute of biotechnology,college of bioscience and biotechnology,national cheng kung university,tainan, Taiwan
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Authors
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|