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Cyclophilin A Associates with Enterovirus-71 Virus Capsid and Plays an Essential Role in Viral Infection as an Uncoating Regulator
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نویسنده
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qing j. ,wang y. ,sun y. ,huang j. ,yan w. ,wang j. ,su d. ,ni c. ,li j. ,rao z. ,liu l. ,lou z.
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منبع
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plos pathogens - 2014 - دوره : 10 - شماره : 10
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چکیده
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Viruses utilize host factors for their efficient proliferation. by evaluating the inhibitory effects of compounds in our library,we identified inhibitors of cyclophilin a (cypa),a known immunosuppressor with peptidyl-prolyl cis-trans isomerase activity,can significantly attenuate ev71 proliferation. we demonstrated that cypa played an essential role in ev71 entry and that the rna interference-mediated reduction of endogenous cypa expression led to decreased ev71 multiplication. we further revealed that cypa directly interacted with and modified the conformation of h-i loop of the vp1 protein in ev71 capsid,and thus regulated the uncoating process of ev71 entry step in a ph-dependent manner. our results aid in the understanding of how host factors influence ev71 life cycle and provide new potential targets for developing antiviral agents against ev71 infection. © 2014 qing et al.
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آدرس
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tsinghua-peking center for life sciences,key laboratory of bioorganic phosphorus chemistry & chemical biology (ministry of education),department of chemistry,tsinghua university,beijing,china,school of medicine,tsinghua university,beijing, China, school of medicine,tsinghua university,beijing,china,national laboratory of macromolecules,institute of biophysics,chinese academy of science,beijing, China, national laboratory of macromolecules,institute of biophysics,chinese academy of science,beijing, China, school of medicine,tsinghua university,beijing, China, school of pharmacy,east china university of science and technology,shanghai, China, school of medicine,tsinghua university,beijing, China, collaborative innovation center for biotherapy,state key laboratory of biotherapy and cancer center,west china hospital,west china medical school,sichuan university,chengdu, China, beijing no. 4 high school,beijing, China, school of pharmacy,east china university of science and technology,shanghai, China, school of medicine,tsinghua university,beijing,china,national laboratory of macromolecules,institute of biophysics,chinese academy of science,beijing, China, tsinghua-peking center for life sciences,key laboratory of bioorganic phosphorus chemistry & chemical biology (ministry of education),department of chemistry,tsinghua university,beijing, China, school of medicine,tsinghua university,beijing,china,collaborative innovation center for biotherapy,state key laboratory of biotherapy and cancer center,west china hospital,west china medical school,sichuan university,chengdu, China
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Authors
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