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Microbial Contamination in Next Generation Sequencing: Implications for Sequence-Based Analysis of Clinical Samples
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نویسنده
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strong m.j. ,xu g. ,morici l. ,splinter bon-durant s. ,baddoo m. ,lin z. ,fewell c. ,taylor c.m. ,flemington e.k.
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منبع
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plos pathogens - 2014 - دوره : 10 - شماره : 11
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چکیده
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The high level of accuracy and sensitivity of next generation sequencing for quantifying genetic material across organismal boundaries gives it tremendous potential for pathogen discovery and diagnosis in human disease. despite this promise,substantial bacterial contamination is routinely found in existing human-derived rna-seq datasets that likely arises from environmental sources. this raises the need for stringent sequencing and analysis protocols for studies investigating sequence-based microbial signatures in clinical samples. © 2014 strong et al.
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آدرس
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department of pathology,tulane university,new orleans,la,united states,tulane cancer center,tulane university,new orleans,la, United States, department of genomic medicine,university of california,san diego,ca, United States, department of microbiology and immunology,tulane university,new orleans,la, United States, university of wisconsin biotechnology center,university of wisconsin,madison,wi, United States, department of pathology,tulane university,new orleans,la,united states,tulane cancer center,tulane university,new orleans,la, United States, department of pathology,tulane university,new orleans,la,united states,tulane cancer center,tulane university,new orleans,la, United States, department of pathology,tulane university,new orleans,la,united states,tulane cancer center,tulane university,new orleans,la, United States, department of microbiology,immunology & parasitology,louisiana state university school of medicine,new orleans,la,united states,research institute for children,children’s hospital of new orleans,new orleans,la, United States, department of pathology,tulane university,new orleans,la,united states,tulane cancer center,tulane university,new orleans,la, United States
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Authors
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