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Murine Anti-vaccinia Virus D8 Antibodies Target Different Epitopes and Differ in Their Ability to Block D8 Binding to CS-E
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نویسنده
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matho m.h. ,de val n. ,miller g.m. ,brown j. ,schlossman a. ,meng x. ,crotty s. ,peters b. ,xiang y. ,hsieh-wilson l.c. ,ward a.b. ,zajonc d.m.
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منبع
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plos pathogens - 2014 - دوره : 10 - شماره : 12
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چکیده
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The imv envelope protein d8 is an adhesion molecule and a major immunodominant antigen of vaccinia virus (vacv). here we identified the optimal d8 ligand to be chondroitin sulfate e (cs-e). cs-e is characterized by a disaccharide moiety with two sulfated hydroxyl groups at positions 4′ and 6′ of galnac. to study the role of antibodies in preventing d8 adhesion to cs-e,we have used a panel of murine monoclonal antibodies,and tested their ability to compete with cs-e for d8 binding. among four antibody specificity groups,mabs of one group (group iv) fully abrogated cs-e binding,while mabs of a second group (group iii) displayed widely varying levels of cs-e blocking. using em,we identified the binding site for each antibody specificity group on d8. recombinant d8 forms a hexameric arrangement,mediated by self-association of a small c-terminal domain of d8. we propose a model in which d8 oligomerization on the imv would allow vacv to adhere to heterogeneous population of cs,including cs-c and potentially cs-a,while overall increasing binding efficiency to cs-e. © 2014 matho et al.
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آدرس
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division of cell biology,la jolla institute for allergy and imunology (liai),la jolla,ca, United States, department of integrative structural and computational biology,the scripps research institute,la jolla,ca, United States, howard hughes medical institute,division of chemistry and chemical engineering,california institute of technology,pasadena,ca, United States, howard hughes medical institute,division of chemistry and chemical engineering,california institute of technology,pasadena,ca, United States, division of cell biology,la jolla institute for allergy and imunology (liai),la jolla,ca, United States, department of microbiology and immunology,university of texas health science center,san antonio,tx, United States, division of vaccine discovery,la jolla institute for allergy and immunology (liai),la jolla,ca, United States, division of vaccine discovery,la jolla institute for allergy and immunology (liai),la jolla,ca, United States, department of microbiology and immunology,university of texas health science center,san antonio,tx, United States, howard hughes medical institute,division of chemistry and chemical engineering,california institute of technology,pasadena,ca, United States, department of integrative structural and computational biology,the scripps research institute,la jolla,ca, United States, division of cell biology,la jolla institute for allergy and imunology (liai),la jolla,ca, United States
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Authors
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