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Six RNA viruses and forty-one hosts: Viral small RNAs and modulation of small RNA repertoires in vertebrate and invertebrate systems
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نویسنده
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parameswaran p. ,sklan e. ,wilkins c. ,burgon t. ,samuel m.a. ,lu r. ,ansel k.m. ,heissmeyer v. ,einav s. ,jackson w. ,doukas t. ,paranjape s. ,polacek c. ,dos santos f.b. ,jalili r. ,babrzadeh f. ,gharizadeh b. ,grimm d. ,kay m. ,koike s. ,sarnow p. ,ronaghi m. ,ding s.-w. ,harris e. ,chow m. ,diamond m.s. ,kirkegaard k. ,glenn j.s. ,fire a.z.
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منبع
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plos pathogens - 2010 - دوره : 6 - شماره : 2
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چکیده
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We have used multiplexed high-throughput sequencing to characterize changes in small rna populations that occur during viral infection in animal cells. small rna-based mechanisms such as rna interference (rnai) have been shown in plant and invertebrate systems to play a key role in host responses to viral infection. although homologs of the key rnai effector pathways are present in mammalian cells,and can launch an rnai-mediated degradation of experimentally targeted mrnas,any role for such responses in mammalian host-virus interactions remains to be characterized. six different viruses were examined in 41 experimentally susceptible and resistant host systems. we identified virus-derived small rnas (vsrnas) from all six viruses,with total abundance varying from vanishingly rare (less than 0.1% of cellular small rna) to highly abundant (comparable to abundant micro-rnas mirnas). in addition to the appearance of vsrnas during infection,we saw a number of specific changes in host mirna profiles. for several infection models investigated in more detail,the rnai and interferon pathways modulated the abundance of vsrnas. we also found evidence for populations of vsrnas that exist as duplexed sirnas with zero to three nucleotide 39 overhangs. using populations of cells carrying a hepatitis c replicon,we observed strand-selective loading of sirnas onto argonaute complexes. these experiments define vsrnas as one possible component of the interplay between animal viruses and their hosts. © 2010 parameswaran et al.
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آدرس
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department of microbiology and immunology,stanford university school of medicine,stanford,ca, United States, department of gastroenterology and hepatology,stanford university school of medicine,stanford,ca,united states,department of clinical microbiology and immunology,tel-aviv university,ramat-aviv,tel-aviv, Israel, department of microbiology and immunology,university of arkansas for medical sciences,little rock,ar,united states,department of immunology,university of washington school of medicine,seattle,wa, United States, department of microbiology and immunology,stanford university school of medicine,stanford,ca,united states,sg2,evanston,il, United States, department of molecular microbiology,washington university school of medicine,st. louis,mo,united states,harvard university,cambridge,ma, United States, department of plant pathology and microbiology,university of california at riverside,riverside,ca,united states,department of biological sciences,louisiana state university,baton rouge,la, United States, strategic asthma basic research center,department of microbiology and immunology,university of california at san francisco,san francisco,ca, United States, institute of molecular immunology,helmholtz center munich,german research center for environmental health,munich, Germany, department of gastroenterology and hepatology,stanford university school of medicine,stanford,ca, United States, department of microbiology and immunology,stanford university school of medicine,stanford,ca,united states,department of microbiology and molecular genetics,center for biopreparedness and infectious diseases,medical college of wisconsin,milwaukee,wi, United States, department of microbiology and immunology,stanford university school of medicine,stanford,ca, United States, division of infectious diseases and vaccinology,school of public health,university of california at berkeley,berkeley,ca,united states,science and technology policy fellowship program,american association for the advancement of sciences,washington,dc, United States, division of infectious diseases and vaccinology,school of public health,university of california at berkeley,berkeley,ca,united states,national veterinary institute,lindholm, Denmark, division of infectious diseases and vaccinology,school of public health,university of california at berkeley,berkeley,ca,united states,laboratório de flavivirus,pav. helio e peggy pereira,instituto oswaldo cruz/fundação oswaldo cruz,rio de janeiro, Brazil, stanford genome technology center,stanford university school of medicine,stanford,ca, United States, stanford genome technology center,stanford university school of medicine,stanford,ca, United States, stanford genome technology center,stanford university school of medicine,stanford,ca, United States, departments of pediatrics and genetics,stanford university school of medicine,stanford,ca,united states,dept. of virology,university of heidelberg,heidelberg, Germany, departments of pediatrics and genetics,stanford university school of medicine,stanford,ca, United States, tokyo metropolitan organization for medical research,tokyo metropolitan institute of medical science,tokyo, Japan, department of microbiology and immunology,stanford university school of medicine,stanford,ca, United States, stanford genome technology center,stanford university school of medicine,stanford,ca,united states,illumina,san diego,ca, United States, department of plant pathology and microbiology,university of california at riverside,riverside,ca, United States, division of infectious diseases and vaccinology,school of public health,university of california at berkeley,berkeley,ca, United States, department of microbiology and immunology,university of arkansas for medical sciences,little rock,ar, United States, departments of medicine,molecular microbiology,pathology and immunology,washington university school of medicine,st. louis,mo, United States, department of microbiology and immunology,stanford university school of medicine,stanford,ca, United States, department of gastroenterology and hepatology,stanford university school of medicine,stanford,ca, United States, departments of pathology and genetics,stanford university school of medicine,stanford,ca, United States
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Authors
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