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A genomic survey of positive selection in Burkholderia pseudomallei provides insights into the evolution of accidental virulence
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نویسنده
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nandi t. ,ong c. ,singh a.p. ,boddey j. ,atkins t. ,sarkar-tyson m. ,essex-lopresti a.e. ,chua h.h. ,pearson t. ,kreisberg j.f. ,nilsson c. ,ariyaratne p. ,ronning c. ,losada l. ,ruan y. ,sung w.-k. ,woods d. ,titball r.w. ,beacham i. ,peak i. ,keim p. ,nierman w.c. ,tan p.
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منبع
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plos pathogens - 2010 - دوره : 6 - شماره : 4 - صفحه:1 -15
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چکیده
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Certain environmental microorganisms can cause severe human infections,even in the absence of an obvious requirement for transition through an animal host for replication (accidental virulence). to understand this process,we compared eleven isolate genomes of burkholderia pseudomallei (bp),a tropical soil microbe and causative agent of the human and animal disease melioidosis. we found evidence for the existence of several new genes in the bp reference genome,identifying 282 novel genes supported by at least two independent lines of supporting evidence (mrna transcripts,database homologs,and presence of ribosomal binding sites) and 81 novel genes supported by all three lines. within the bp core genome,211 genes exhibited significant levels of positive selection (4.5%),distributed across many cellular pathways including carbohydrate and secondary metabolism. functional experiments revealed that certain positively selected genes might enhance mammalian virulence by interacting with host cellular pathways or utilizing host nutrients. evolutionary modifications improving bp environmental fitness may thus have indirectly facilitated the ability of bp to colonize and survive in mammalian hosts. these findings improve our understanding of the pathogenesis of melioidosis,and establish bp as a model system for studying the genetics of accidental virulence.
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آدرس
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genome institute of singapore,singapore, Singapore, defense medical and environmental research institute,dso national laboratories,singapore, Singapore, genome institute of singapore,singapore, Singapore, institute for glycomics,griffith university,gold coast campus,southport,qld, Australia, defence science and technology laboratory,porton down,salisbury, United Kingdom, defence science and technology laboratory,porton down,salisbury, United Kingdom, defence science and technology laboratory,porton down,salisbury, United Kingdom, genome institute of singapore,singapore, Singapore, microbial genetics and genomics,northern arizona university,flagstaff,az, United States, genome institute of singapore,singapore, Singapore, genome institute of singapore,singapore, Singapore, genome institute of singapore,singapore, Singapore, j. craig venter institute,rockville,md, United States, j. craig venter institute,rockville,md, United States, genome institute of singapore,singapore, Singapore, genome institute of singapore,singapore, Singapore, university of calgary health sciences centre,calgary,ab, Canada, defence science and technology laboratory,porton down,salisbury,united kingdom,school of biosciences,university of exeter,exeter, United Kingdom, institute for glycomics,griffith university,gold coast campus,southport,qld, Australia, institute for glycomics,griffith university,gold coast campus,southport,qld, Australia, microbial genetics and genomics,northern arizona university,flagstaff,az, United States, j. craig venter institute,rockville,md, United States, genome institute of singapore,singapore,singapore,duke-nus graduate medical school singapore,singapore, Singapore
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Authors
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