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Persistent growth of a human plasma-derived hepatitis C virus genotype 1b isolate in cell culture
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نویسنده
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silberstein e. ,mihalik k. ,ulitzky l. ,plant e.p. ,puig m. ,gagneten s. ,yu m.-y.w. ,kaushik-basu n. ,feinstone s.m. ,taylor d.r.
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منبع
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plos pathogens - 2010 - دوره : 6 - شماره : 5 - صفحه:1 -13
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چکیده
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Hcv (hepatitis c virus) research,including therapeutics and vaccine development,has been hampered by the lack of suitable tissue culture models. development of cell culture systems for the growth of the most drug-resistant hcv genotype (1b) as well as natural isolates has remained a challenge. transfection of cultured cells with adenovirus-associated rnai (va rnai),a known interferon (ifn) antagonist and inhibitor of dsrna-mediated antiviral pathways,enhanced the growth of plasma-derived hcv genotype 1b. furthermore,persistent viral growth was achieved after passaging through ifn-α/βdeficient veroe6 cells for 2 years. persistently infected cells were maintained in culture for an additional 4 years,and the virus rescued from these cells induced strong cytopathic effect (cpe). using a cpe-based assay,we measured inhibition of viral production by anti-hcv specific inhibitors,including 29-c-methyl-d-adenosine,demonstrating its utility for the evaluation of hcv antivirals. this virus constitutes a novel tool for the study of one of the most relevant strains of hcv,genotype 1b,which will now be available for hcv life cycle research and useful for the development of new therapeutics.
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آدرس
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division of emerging and transfusion-transmitted diseases,center for biologics evaluation and research,food and drug administration,bethesda,md, United States, division of viral products,center for biologics evaluation and research,food and drug administration,bethesda,md, United States, division of emerging and transfusion-transmitted diseases,center for biologics evaluation and research,food and drug administration,bethesda,md, United States, division of emerging and transfusion-transmitted diseases,center for biologics evaluation and research,food and drug administration,bethesda,md, United States, division of therapeutic proteins,center for drug evaluation and research,food and drug administration,bethesda,md, United States, division of viral products,center for biologics evaluation and research,food and drug administration,bethesda,md, United States, division of hematology,center for biologics evaluation and research,food and drug administration,bethesda,md, United States, department of biochemistry and molecular biology,university of medicine and dentistry of new jersey,newark,nj, United States, division of viral products,center for biologics evaluation and research,food and drug administration,bethesda,md, United States, division of emerging and transfusion-transmitted diseases,center for biologics evaluation and research,food and drug administration,bethesda,md, United States
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Authors
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