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A viral microRNA down-regulates multiple cell cycle genes through mRNA 59′UTRs
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نویسنده
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grey f. ,tirabassi r. ,meyers h. ,wu g. ,mcweeney s. ,hook l. ,nelson j.a.
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منبع
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plos pathogens - 2010 - دوره : 6 - شماره : 6
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چکیده
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Global gene expression data combined with bioinformatic analysis provides strong evidence that mammalian mirnas mediate repression of gene expression primarily through binding sites within the 39 untranslated region (utr). using rna induced silencing complex immunoprecipitation (risc-ip) techniques we have identified multiple cellular targets for a human cytomegalovirus (hcmv) mirna,mir-us25-1. strikingly,this mirna binds target sites primarily within 59′utrs,mediating significant reduction in gene expression. intriguingly,many of the genes targeted by mir-us25-1 are associated with cell cycle control,including cyclin e2,brcc3,eid1,mapre2,and cd147,suggesting that mir-us25-1 is targeting genes within a related pathway. deletion of mir-us25-1 from hcmv results in over expression of cyclin e2 in the context of viral infection. our studies demonstrate that a viral mirna mediates translational repression of multiple cellular genes by targeting mrna 59′utrs. © 2010 grey et al.
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آدرس
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vaccine and gene therapy institute,oregon health and science university,portland,or,united states,centre for infectious diseases,the roslin institute and royal (dick) school,university of edinburgh,summerhall,edinburgh, United Kingdom, vaccine and gene therapy institute,oregon health and science university,portland,or, United States, vaccine and gene therapy institute,oregon health and science university,portland,or, United States, octri,oregon health and science university,portland,or, United States, knight cancer institute,oregon health and science university,portland,or, United States, vaccine and gene therapy institute,oregon health and science university,portland,or, United States, vaccine and gene therapy institute,oregon health and science university,portland,or, United States
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Authors
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