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Transcription and translation products of the cytolysin gene psm-mec on the mobile genetic element SCCmec regulate Staphylococcus aureus virulence
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نویسنده
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kaito c. ,saito y. ,nagano g. ,ikuo m. ,omae y. ,hanada y. ,han x. ,kuwahara-arai k. ,hishinuma t. ,baba t. ,ito t. ,hiramatsu k. ,sekimizu k.
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منبع
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plos pathogens - 2011 - دوره : 7 - شماره : 2
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چکیده
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The f region downstream of the meci gene in the sccmec element in hospital-associated methicillin-resistant staphylococcus aureus (ha-mrsa) contains two bidirectionally overlapping open reading frames (orfs),the fudoh orf and the psm-mec orf. the psm-mec orf encodes a cytolysin,phenol-soluble modulin (psm)-mec. transformation of the f region into the newman strain,which is a methicillin-sensitive s. aureus (mssa) strain,or into the mw2 (usa400) and frp3757 (usa300) strains,which are community-acquired mrsa (ca-mrsa) strains that lack the f region,attenuated their virulence in a mouse systemic infection model. introducing the f region to these strains suppressed colony-spreading activity and psmα production,and promoted biofilm formation. by producing mutations into the psm-mec orf,we revealed that (i) both the transcription and translation products of the psm-mec orf suppressed colony-spreading activity and promoted biofilm formation; and (ii) the transcription product of the psm-mec orf,but not its translation product,decreased psmα production. these findings suggest that both the psm-mec transcript,acting as a regulatory rna,and the psm-mec protein encoded by the gene on the mobile genetic element sccmec regulate the virulence of staphylococcus aureus. © 2011 kaito et al.
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آدرس
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laboratory of microbiology,graduate school of pharmaceutical sciences,the university of tokyo,tokyo, Japan, laboratory of microbiology,graduate school of pharmaceutical sciences,the university of tokyo,tokyo, Japan, laboratory of microbiology,graduate school of pharmaceutical sciences,the university of tokyo,tokyo, Japan, laboratory of microbiology,graduate school of pharmaceutical sciences,the university of tokyo,tokyo, Japan, laboratory of microbiology,graduate school of pharmaceutical sciences,the university of tokyo,tokyo, Japan, laboratory of microbiology,graduate school of pharmaceutical sciences,the university of tokyo,tokyo, Japan, department of infection control science,graduate school of medicine,juntendo university,tokyo, Japan, department of infection control science,graduate school of medicine,juntendo university,tokyo, Japan, department of infection control science,graduate school of medicine,juntendo university,tokyo, Japan, department of infection control science,graduate school of medicine,juntendo university,tokyo, Japan, department of infection control science,graduate school of medicine,juntendo university,tokyo, Japan, department of infection control science,graduate school of medicine,juntendo university,tokyo, Japan, laboratory of microbiology,graduate school of pharmaceutical sciences,the university of tokyo,tokyo, Japan
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Authors
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