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Dynamic imaging of the effector immune response to listeria infection in Vivo
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نویسنده
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waite j.c. ,leiner i. ,lauer p. ,rae c.s. ,barbet g. ,zheng h. ,portnoy d.a. ,pamer e.g. ,dustin m.l.
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منبع
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plos pathogens - 2011 - دوره : 7 - شماره : 3
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چکیده
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Host defense against the intracellular pathogen listeria monocytogenes (lm) requires innate and adaptive immunity. here,we directly imaged immune cell dynamics at lm foci established by dendritic cells in the subcapsular red pulp (scdc) using intravital microscopy. blood borne lm rapidly associated with scdc. myelomonocytic cells (mmc) swarmed around non-motile scdc forming foci from which blood flow was excluded. the depletion of scdc after foci were established resulted in a 10-fold reduction in viable lm,while graded depletion of mmc resulted in 30-1000 fold increase in viable lm in foci with enhanced blood flow. effector cd8+ t cells at sites of infection displayed a two-tiered reduction in motility with antigen independent and antigen dependent components,including stable interactions with infected and non-infected scdc. thus,swarming mmc contribute to control of lm prior to development of t cell immunity by direct killing and sequestration from blood flow,while scdc appear to promote lm survival while preferentially interacting with cd8+ t cells in effector sites. © 2011 waite et al.
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آدرس
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program in molecular pathogenesis,helen l. and martin s. kimmel center for biology and medicine,skirball institute of biomolecular medicine,new york university school of medicine,new york city,ny, United States, infectious disease service,department of medicine,memorial sloan-kettering cancer center,immunology program,sloan-kettering institute,new york city,ny, United States, aduro biotech,berkeley,ca, United States, department of molecular and cell biology,university of california,berkeley,berkeley,ca, United States, program in molecular pathogenesis,helen l. and martin s. kimmel center for biology and medicine,skirball institute of biomolecular medicine,new york university school of medicine,new york city,ny, United States, department of chemical engineering,massachusetts institute of technology,cambridge,ma, United States, department of molecular and cell biology,university of california,berkeley,berkeley,ca,united states,school of public health,university of california,berkeley,berkeley,ca, United States, infectious disease service,department of medicine,memorial sloan-kettering cancer center,immunology program,sloan-kettering institute,new york city,ny, United States, program in molecular pathogenesis,helen l. and martin s. kimmel center for biology and medicine,skirball institute of biomolecular medicine,new york university school of medicine,new york city,ny, United States
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Authors
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