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Legionella pneumophila secretes a mitochondrial carrier protein during infection
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نویسنده
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dolezal p. ,aili m. ,tong j. ,jiang j.-h. ,marobbio c.m. ,lee s.f. ,schuelein r. ,belluzzo s. ,binova e. ,mousnier a. ,frankel g. ,giannuzzi g. ,palmieri f. ,gabriel k. ,naderer t. ,hartland e.l. ,lithgow t.
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منبع
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plos pathogens - 2012 - دوره : 8 - شماره : 1
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چکیده
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The mitochondrial carrier family (mcf) is a signature group of integral membrane proteins that transport metabolites across the mitochondrial inner membrane in eukaryotes. mcf proteins are characterized by six transmembrane segments that assemble to form a highly-selective channel for metabolite transport. we discovered a novel mcf member,termed legionella nucleotide carrier protein (lncp),encoded in the genome of legionella pneumophila,the causative agent of legionnaire's disease. lncp was secreted via the bacterial dot/icm type iv secretion system into macrophages and assembled in the mitochondrial inner membrane. in a yeast cellular system,lncp induced a dominant-negative phenotype that was rescued by deleting an endogenous atp carrier. substrate transport studies on purified lncp reconstituted in liposomes revealed that it catalyzes unidirectional transport and exchange of atp transport across membranes,thereby supporting a role for lncp as an atp transporter. a hidden markov model revealed further mcf proteins in the intracellular pathogens,legionella longbeachae and neorickettsia sennetsu,thereby challenging the notion that mcf proteins exist exclusively in eukaryotic organisms. © 2012 dolezal et al.
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آدرس
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department of biochemistry and molecular biology,monash university,clayton,australia,department of parasitology,charles university,prague, Czech Republic, department of microbiology and immunology,university of melbourne,parkville,australia,uppsala biocenter,department of plant biology and forest genetics,the swedish university of agricultural sciences (slu),uppsala, Sweden, department of biochemistry and molecular biology,monash university,clayton, Australia, department of biochemistry and molecular biology,monash university,clayton,australia,department of microbiology and immunology,university of melbourne,parkville, Australia, department of pharmaco-biology,laboratory of biochemistry and molecular biology,university of bari,bari, Italy, department of microbiology and immunology,university of melbourne,parkville, Australia, department of microbiology and immunology,university of melbourne,parkville, Australia, department of microbiology and immunology,university of melbourne,parkville, Australia, department of tropical medicine,1st faculty of medicine,charles university in prague and faculty hospital bulovka,prague, Czech Republic, centre for molecular microbiology and infection,division of cell and molecular biology,imperial college london,london, United Kingdom, centre for molecular microbiology and infection,division of cell and molecular biology,imperial college london,london, United Kingdom, department of pharmaco-biology,laboratory of biochemistry and molecular biology,university of bari,bari, Italy, department of pharmaco-biology,laboratory of biochemistry and molecular biology,university of bari,bari, Italy, department of biochemistry and molecular biology,monash university,clayton, Australia, department of biochemistry and molecular biology,monash university,clayton, Australia, department of microbiology and immunology,university of melbourne,parkville, Australia, department of biochemistry and molecular biology,monash university,clayton, Australia
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Authors
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