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Structure-function analysis of barley NLR immune receptor MLA10 reveals its cell compartment specific activity in cell death and disease resistance
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نویسنده
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bai s. ,liu j. ,chang c. ,zhang l. ,maekawa t. ,wang q. ,xiao w. ,liu y. ,chai j. ,takken f.l.w. ,schulze-lefert p. ,shen q.-h.
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منبع
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plos pathogens - 2012 - دوره : 8 - شماره : 6
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چکیده
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Plant intracellular immune receptors comprise a large number of multi-domain proteins resembling animal nod-like receptors (nlrs). plant nlrs typically recognize isolate-specific pathogen-derived effectors,encoded by avirulence (avr) genes,and trigger defense responses often associated with localized host cell death. the barley mla gene is polymorphic in nature and encodes nlrs of the coiled-coil (cc)-nb-lrr type that each detects a cognate isolate-specific effector of the barley powdery mildew fungus. we report the systematic analyses of mla10 activity in disease resistance and cell death signaling in barley and nicotiana benthamiana. mla10 cc domain-triggered cell death is regulated by highly conserved motifs in the cc and the nb-arc domains and by the c-terminal lrr of the receptor. enforced mla10 subcellular localization,by tagging with a nuclear localization sequence (nls) or a nuclear export sequence (nes),shows that mla10 activity in cell death signaling is suppressed in the nucleus but enhanced in the cytoplasm. by contrast,nuclear localized mla10 is sufficient to mediate disease resistance against powdery mildew fungus. mla10 retention in the cytoplasm was achieved through attachment of a glucocorticoid receptor hormone-binding domain (gr),by which we reinforced the role of cytoplasmic mla10 in cell death signaling. together with our data showing an essential and sufficient nuclear mla10 activity in disease resistance,this suggests a bifurcation of mla10-triggered cell death and disease resistance signaling in a compartment-dependent manner. © 2012 bai et al.
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آدرس
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state key laboratory of plant cell and chromosome engineering,institute of genetics and developmental biology,chinese academy of sciences,beijing,china,graduate university of chinese academy of sciences,beijing, China, state key laboratory of plant cell and chromosome engineering,institute of genetics and developmental biology,chinese academy of sciences,beijing,china,graduate university of chinese academy of sciences,beijing, China, state key laboratory of plant cell and chromosome engineering,institute of genetics and developmental biology,chinese academy of sciences,beijing,china,graduate university of chinese academy of sciences,beijing, China, state key laboratory of plant cell and chromosome engineering,institute of genetics and developmental biology,chinese academy of sciences,beijing, China, department of plant microbe interactions,max-planck institut pflanzenzüchtungsforschung,cologne, Germany, state key laboratory of plant cell and chromosome engineering,institute of genetics and developmental biology,chinese academy of sciences,beijing, China, state key laboratory of plant cell and chromosome engineering,institute of genetics and developmental biology,chinese academy of sciences,beijing, China, school of life sciences,tsinghua university,beijing, China, school of life sciences,tsinghua university,beijing, China, molecular plant pathology,swammerdam institute for life sciences,university of amsterdam,amsterdam,netherlands,centre for biosystem genomics,wageningen, Netherlands, department of plant microbe interactions,max-planck institut pflanzenzüchtungsforschung,cologne, Germany, state key laboratory of plant cell and chromosome engineering,institute of genetics and developmental biology,chinese academy of sciences,beijing, China
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Authors
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