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The ESCRT Machinery Is Recruited by the Viral BFRF1 Protein to the Nucleus-Associated Membrane for the Maturation of Epstein-Barr Virus
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نویسنده
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lee c.-p. ,liu p.-t. ,kung h.-n. ,su m.-t. ,chua h.-h. ,chang y.-h. ,chang c.-w. ,tsai c.-h. ,liu f.-t. ,chen m.-r.
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منبع
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plos pathogens - 2012 - دوره : 8 - شماره : 9
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چکیده
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The cellular endosomal sorting complex required for transport (escrt) machinery participates in membrane scission and cytoplasmic budding of many rna viruses. here,we found that expression of dominant negative escrt proteins caused a blockade of epstein-barr virus (ebv) release and retention of viral bfrf1 at the nuclear envelope. the escrt adaptor protein alix was redistributed and partially colocalized with bfrf1 at the nuclear rim of virus replicating cells. following transient transfection,bfrf1 associated with escrt proteins,reorganized the nuclear membrane and induced perinuclear vesicle formation. multiple domains within bfrf1 mediated vesicle formation and alix recruitment,whereas both bro and prr domains of alix interacted with bfrf1. inhibition of escrt machinery abolished bfrf1-induced vesicle formation,leading to the accumulation of viral dna and capsid proteins in the nucleus of ebv-replicating cells. overall,data here suggest that bfrf1 recruits the escrt components to modulate nuclear envelope for the nuclear egress of ebv. © 2012 lee et al.
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آدرس
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graduate institute and department of microbiology,college of medicine,national taiwan university,taipei,taiwan,center of general education,national taipei university of nursing and health sciences,taipei, Taiwan, graduate institute and department of microbiology,college of medicine,national taiwan university,taipei, Taiwan, department of anatomy and cell biology,college of medicine,national taiwan university,taipei, Taiwan, graduate institute and department of microbiology,college of medicine,national taiwan university,taipei, Taiwan, graduate institute and department of microbiology,college of medicine,national taiwan university,taipei, Taiwan, graduate institute and department of microbiology,college of medicine,national taiwan university,taipei, Taiwan, graduate institute and department of microbiology,college of medicine,national taiwan university,taipei, Taiwan, graduate institute and department of microbiology,college of medicine,national taiwan university,taipei, Taiwan, institute of biomedical sciences,academia sinica,taipei,taiwan,department of dermatology,school of medicine,university of california,davis,ca, United States, graduate institute and department of microbiology,college of medicine,national taiwan university,taipei, Taiwan
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Authors
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