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Antibody-Mediated Neutralization of the Exotoxin Mycolactone,the Main Virulence Factor Produced by Mycobacterium ulcerans
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نویسنده
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dangy j.-p. ,scherr n. ,gersbach p. ,hug m.n. ,bieri r. ,bomio c. ,li j. ,huber s. ,altmann k.-h. ,pluschke g.
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منبع
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plos neglected tropical diseases - 2016 - دوره : 10 - شماره : 6
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چکیده
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Background: mycolactone,the macrolide exotoxin produced by mycobacterium ulcerans,causes extensive tissue destruction by inducing apoptosis of host cells. in this study,we aimed at the production of antibodies that could neutralize the cytotoxic activities of mycolactone. methodology/principal findings: using the b cell hybridoma technology,we generated a series of monoclonal antibodies with specificity for mycolactone from spleen cells of mice immunized with the protein conjugate of a truncated synthetic mycolactone derivative. l929 fibroblasts were used as a model system to investigate whether these antibodies can inhibit the biological effects of mycolactone. by measuring the metabolic activity of the fibroblasts,we found that anti-mycolactone mabs can completely neutralize the cytotoxic activity of mycolactone. conclusions/significance: the toxin neutralizing capacity of anti-mycolactone mabs supports the concept of evaluating the macrolide toxin as vaccine target. © 2016 dangy et al.
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آدرس
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swiss tropical and public health institute,basel,switzerland,university of basel,basel, Switzerland, swiss tropical and public health institute,basel,switzerland,university of basel,basel, Switzerland, department of chemistry and applied biosciences,institute of pharmaceutical sciences,swiss federal institute of technology (eth) zürich,zürich, Switzerland, roche innovation center,chemical biology,basel, Switzerland, swiss tropical and public health institute,basel,switzerland,university of basel,basel, Switzerland, department of chemistry and applied biosciences,institute of pharmaceutical sciences,swiss federal institute of technology (eth) zürich,zürich, Switzerland, department of chemistry and applied biosciences,institute of pharmaceutical sciences,swiss federal institute of technology (eth) zürich,zürich, Switzerland, roche innovation center,chemical biology,basel, Switzerland, department of chemistry and applied biosciences,institute of pharmaceutical sciences,swiss federal institute of technology (eth) zürich,zürich, Switzerland, swiss tropical and public health institute,basel,switzerland,university of basel,basel, Switzerland
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Authors
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