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West Nile Virus Temperature Sensitivity and Avian Virulence Are Modulated by NS1-2B Polymorphisms
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نویسنده
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dietrich e.a. ,langevin s.a. ,huang c.y.h. ,maharaj p.d. ,delorey m.j. ,bowen r.a. ,kinney r.m. ,brault a.c.
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منبع
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plos neglected tropical diseases - 2016 - دوره : 10 - شماره : 8
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چکیده
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West nile virus (wnv) replicates in a wide variety of avian species,which serve as reservoir and amplification hosts. wnv strains isolated in north america,such as the prototype strain ny99,elicit a highly pathogenic response in certain avian species,notably american crows (amcrs; corvus brachyrhynchos). in contrast,a closely related strain,kn3829,isolated in kenya,exhibits a low viremic response with limited mortality in amcrs. previous work has associated the difference in pathogenicity primarily with a single amino acid mutation at position 249 in the helicase domain of the ns3 protein. the ny99 strain encodes a proline residue at this position,while kn3829 encodes a threonine. introduction of an ns3-t249p mutation in the kn3829 genetic background significantly increased virulence and mortality; however,peak viremia and mortality were lower than those of ny99. in order to elucidate the viral genetic basis for phenotype variations exclusive of the ns3-249 polymorphism,chimeric ny99/kn3829 viruses were created. we show herein that differences in the ns1-2b region contribute to avian pathogenicity in a manner that is independent of and additive with the ns3-249 mutation. additionally,ns1-2b residues were found to alter temperature sensitivity when grown in avian cells. © 2016,public library of science. all rights reserved.
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آدرس
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division of vector-borne diseases,centers for disease control and prevention,fort collins,co, United States, department of pathology,microbiology and immunology,school of veterinary medicine,university of california,davis,davis,ca, United States, division of vector-borne diseases,centers for disease control and prevention,fort collins,co, United States, department of pathology,microbiology and immunology,school of veterinary medicine,university of california,davis,davis,ca, United States, division of vector-borne diseases,centers for disease control and prevention,fort collins,co, United States, department of biomedical sciences,colorado state university,fort collins,co, United States, division of vector-borne diseases,centers for disease control and prevention,fort collins,co, United States, division of vector-borne diseases,centers for disease control and prevention,fort collins,co, United States
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Authors
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