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Aedes aegypti D7 Saliva Protein Inhibits Dengue Virus Infection
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نویسنده
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conway m.j. ,londono-renteria b. ,troupin a. ,watson a.m. ,klimstra w.b. ,fikrig e. ,colpitts t.m.
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منبع
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plos neglected tropical diseases - 2016 - دوره : 10 - شماره : 9
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چکیده
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Aedes aegypti is the primary vector of several medically relevant arboviruses including dengue virus (denv) types 1–4. ae. aegypti transmits denv by inoculating virus-infected saliva into host skin during probing and feeding. ae. aegypti saliva contains over one hundred unique proteins and these proteins have diverse functions,including facilitating blood feeding. previously,we showed that ae. aegypti salivary gland extracts (sges) enhanced dissemination of denv to draining lymph nodes. in contrast,hplc-fractionation revealed that some sge components inhibited infection. here,we show that d7 proteins are enriched in hplc fractions that are inhibitory to denv infection,and that recombinant d7 protein can inhibit denv infection in vitro and in vivo. further,binding assays indicate that d7 protein can directly interact with denv virions and recombinant denv envelope protein. these data reveal a novel role for d7 proteins,which inhibits arbovirus transmission to vertebrates through a direct interaction with virions. © 2016 conway et al.
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آدرس
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foundational sciences,central michigan university college of medicine,mt. pleasant,mi, United States, department of pathology,microbiology and immunology,university of south carolina school of medicine,columbia,sc, United States, department of pathology,microbiology and immunology,university of south carolina school of medicine,columbia,sc, United States, center for vaccine research and department of microbiology and molecular genetics,university of pittsburgh,pittsburgh,pa, United States, center for vaccine research and department of microbiology and molecular genetics,university of pittsburgh,pittsburgh,pa, United States, section of infectious diseases,department of internal medicine,yale university school of medicine,new haven,ct,united states,howard hughes medical institute,chevy chase,md, United States, department of pathology,microbiology and immunology,university of south carolina school of medicine,columbia,sc, United States
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Authors
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