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MLST-based population genetic analysis in a global context reveals clonality amongst Cryptococcus neoformans var. grubii VNI isolates from HIV patients in southeastern Brazil
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نویسنده
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ferreira-paim k. ,andrade-silva l. ,fonseca f.m. ,ferreira t.b. ,mora d.j. ,andrade-silva j. ,khan a. ,dao a. ,reis e.c. ,almeida m.t.g. ,maltos a. ,junior v.r. ,trilles l. ,rickerts v. ,chindamporn a. ,sykes j.e. ,cogliati m. ,nielsen k. ,boekhout t. ,fisher m. ,kwon-chung j. ,engelthaler d.m. ,lazéra m. ,meyer w. ,silva-vergara m.l.
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منبع
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plos neglected tropical diseases - 2017 - دوره : 11 - شماره : 1
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چکیده
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Cryptococcosis is an important fungal infection in immunocompromised individuals,especially those infected with hiv. in brazil,despite the free availability of antiretroviral therapy (art) in the public health system,the mortality rate due to cryptococcus neoformans meningitis is still high. to obtain a more detailed picture of the population genetic structure of this species in southeast brazil,we studied 108 clinical isolates from 101 patients and 35 environmental isolates. among the patients,59% had a fatal outcome mainly in hiv-positive male patients. all the isolates were found to be c. neoformans var. grubii major molecular type vni and mating type locus alpha. twelve were identified as diploid by flow cytometry,being homozygous (aαaα) for the mating type and by pcr screening of the ste20,gpa1,and pak1 genes. using the isham consensus multilocus sequence typing (mlst) scheme,13 sequence types (st) were identified,with one being newly described. st93 was identified from 81 (75%) of the clinical isolates,while st77 and st93 were identified from 19 (54%) and 10 (29%) environmental isolates,respectively. the southeastern brazilian isolates had an overwhelming clonal population structure. when compared with populations from different continents based on data extracted from the isham-mlst database (mlst.mycologylab.org) they showed less genetic variability. two main clusters within c. neoformans var. grubii vni were identified that diverged from vnb around 0.58 to 4.8 million years ago. © 2017,public library of science. all rights reserved.
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آدرس
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molecular mycology research laboratory,centre for infectious diseases and microbiology marie bashir institute for emerging infectious diseases and biosecurity,sydney medical school-westmead hospital,westmead institute for medical research,the university of sydney,sydney,australia,infectious disease department,triangulo mineiro federal university,uberaba, Brazil, infectious disease department,triangulo mineiro federal university,uberaba, Brazil, biomedicine department,federal university of piauí,parnaíba, Brazil, infectious disease department,triangulo mineiro federal university,uberaba, Brazil, infectious disease department,triangulo mineiro federal university,uberaba, Brazil, infectious disease department,triangulo mineiro federal university,uberaba, Brazil, molecular mycology research laboratory,centre for infectious diseases and microbiology marie bashir institute for emerging infectious diseases and biosecurity,sydney medical school-westmead hospital,westmead institute for medical research,the university of sydney,sydney, Australia, molecular mycology research laboratory,centre for infectious diseases and microbiology marie bashir institute for emerging infectious diseases and biosecurity,sydney medical school-westmead hospital,westmead institute for medical research,the university of sydney,sydney, Australia, infectious disease department,faculty of medicine of são josé do rio preto,são josé do rio preto, Brazil, infectious disease department,faculty of medicine of são josé do rio preto,são josé do rio preto, Brazil, infectious disease department,triangulo mineiro federal university,uberaba, Brazil, infectious disease department,triangulo mineiro federal university,uberaba, Brazil, evandro chagas national institute of infectious diseases,oswaldo cruz foundation,rio de janeiro, Brazil, mycology section,robert koch institute,berlin, Germany, mycology unit department of microbiology,faculty of medicine,chulalongkorn university,bangkok, Thailand, department of medicine and epidemiology,university of california,davis, United States, laboratorio micologia medica,dip. scienze biomediche per la salute,università degli studi di milano,milano, Italy, department of microbiology and immunology,medical school university of minnesota,minneapolis,ms, United States, department of yeast and basidiomycete research,cbs-knaw fungal biodiversity centre,utrecht, Netherlands, department of infectious disease epidemiology,imperial college london,norfolk place,london, United Kingdom, molecular microbiology section,laboratory of clinical infectious diseases national institute of allergy and infectious diseases,national institute of health,bethesda,md, United States, translational genomics research institute,flagstaff,az, United States, evandro chagas national institute of infectious diseases,oswaldo cruz foundation,rio de janeiro, Brazil, molecular mycology research laboratory,centre for infectious diseases and microbiology marie bashir institute for emerging infectious diseases and biosecurity,sydney medical school-westmead hospital,westmead institute for medical research,the university of sydney,sydney, Australia, infectious disease department,triangulo mineiro federal university,uberaba, Brazil
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Authors
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